{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Negatu DA"],"pubmed_abstract":["<b>Background</b>: Infections caused by the multidrug-resistant pathogen <i>Mycobacterium abscessus</i> (<i>Mab</i>) are notoriously difficult to treat. The novel β-lactamase inhibitor durlobactam, in combination with β-lactams, shows potent bactericidal activity against <i>Mab</i>, but the potential for acquired resistance remains a clinical concern. <b>Objectives</b>: To identify and characterize mechanisms of acquired resistance to durlobactam in <i>Mab</i>. <b>Methods</b>: In vitro single-step resistance selection was performed by plating wild-type <i>Mab</i> ATCC 19977 and by transcriptional silencing using a CRISPR interference (CRISPRi) system. Minimum inhibitory concentrations (MICs) were determined by both an agar-based method and broth microdilution. <b>Results</b>: Whole-genome "],"journal":["Antibiotics (Basel, Switzerland)"],"pagination":["7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12837516"],"repository":["biostudies-literature"],"pubmed_title":["Loss-of-Function Mutations in the Penicillin-Binding Protein PonA1 Confer Agar-Dependent Resistance to Durlobactam in <i>Mycobacterium abscessus</i>."],"pmcid":["PMC12837516"],"pubmed_authors":["Aragaw WW","Dartois V","Xie M","Negatu DA","Dick T"],"additional_accession":[]},"is_claimable":false,"name":"Loss-of-Function Mutations in the Penicillin-Binding Protein PonA1 Confer Agar-Dependent Resistance to Durlobactam in <i>Mycobacterium abscessus</i>.","description":"<b>Background</b>: Infections caused by the multidrug-resistant pathogen <i>Mycobacterium abscessus</i> (<i>Mab</i>) are notoriously difficult to treat. The novel β-lactamase inhibitor durlobactam, in combination with β-lactams, shows potent bactericidal activity against <i>Mab</i>, but the potential for acquired resistance remains a clinical concern. <b>Objectives</b>: To identify and characterize mechanisms of acquired resistance to durlobactam in <i>Mab</i>. <b>Methods</b>: In vitro single-step resistance selection was performed by plating wild-type <i>Mab</i> ATCC 19977 and by transcriptional silencing using a CRISPR interference (CRISPRi) system. Minimum inhibitory concentrations (MICs) were determined by both an agar-based method and broth microdilution. <b>Results</b>: Whole-genome ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-15T03:14:18.89Z","creation":"2026-06-15T03:08:54.791Z"},"accession":"S-EPMC12837516","cross_references":{"pubmed":["41594045"],"doi":["10.3390/antibiotics15010007"]}}