biostudies-literatureWang XYuanping WangXiao Wang40https://www.ebi.ac.uk/biostudies/studies/S-EPMC12837633biostudies-literatureUnknown15(1)<b>Background:</b> In China, Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) is dominated by sequence type 11 (ST11) harbouring KPC-2, with KL64 displacing KL47 and KL25 emerging. ST859 (ST11 variant) has caused outbreaks, but its epidemiology is unclear. <b>Materials and Methods:</b> A total of 99 non-duplicate CRKP isolates were collected from June to December 2024. Antimicrobial susceptibility was determined by broth microdilution. The genomic sequences of the strains were obtained using next-generation sequencing technology. Resistance genes, virulence loci, and plasmid replicons were identified with Kleborate, Abricate, and MOB-suite, respectively. <b>Results:</b> ST11 accounted for 63.64% and ST859 for 15.15%. All ST859 were KL19, while ST11 were mainly KL25 (60.32%) and KL6Antibiotics (Basel, Switzerland)Comparative Genomic and Resistance Analysis of ST859-KL19 and ST11 Carbapenem-Resistant <i>Klebsiella pneumoniae</i> with Diverse Capsular Serotypes.PMC12837633PWGw2025-05PWRq2025-03Qiu YFeng JRen LZhao BZhang YLiang YYang BWang XWang YfalseComparative Genomic and Resistance Analysis of ST859-KL19 and ST11 Carbapenem-Resistant <i>Klebsiella pneumoniae</i> with Diverse Capsular Serotypes.<b>Background:</b> In China, Carbapenem-resistant <i>Klebsiella pneumoniae</i> (CRKP) is dominated by sequence type 11 (ST11) harbouring KPC-2, with KL64 displacing KL47 and KL25 emerging. ST859 (ST11 variant) has caused outbreaks, but its epidemiology is unclear. <b>Materials and Methods:</b> A total of 99 non-duplicate CRKP isolates were collected from June to December 2024. Antimicrobial susceptibility was determined by broth microdilution. The genomic sequences of the strains were obtained using next-generation sequencing technology. Resistance genes, virulence loci, and plasmid replicons were identified with Kleborate, Abricate, and MOB-suite, respectively. <b>Results:</b> ST11 accounted for 63.64% and ST859 for 15.15%. All ST859 were KL19, while ST11 were mainly KL25 (60.32%) and KL62026-01-01T00:00:00Z2026 Jan2026-06-09T03:17:21.727Z2026-06-09T03:11:56.293ZS-EPMC128376334159407710.3390/antibiotics15010040