{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu Z"],"funding":["Daqing Gao","Lu Chen","Ruihua Shi"],"pagination":["152"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12839154"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["Adherent-invasive <i>E. coli</i> (AIEC) is closely related to inflammatory bowel disease (IBD). However, its pathogenic mechanism has not yet been fully elucidated. Using a BLASTP search, we discovered that the amino acid sequence of a putative protein (UFP37798.1) in the AIEC LF82 strain is highly homologous to some regulators in the SlyA family. We named it EruA. We displayed the secondary structures of EruA using bioinformatics, overexpressed the His<sub>6</sub>-tagged EruA protein using SDS-PAGE, and dissected the genetic organization of the <i>eruA</i> chromosomal region using 5'RACE. We constructed an <i>eruA</i> deletion mutant (ΔeruA) and a complementary strain (CΔeruA) of the LF82 strain. The transcriptomes of wild-type (WT) and ΔeruA bacteria were compared using RNA sequencing an"],"journal":["Biomolecules"],"pubmed_title":["EruA, a Regulator of Adherent-Invasive <i>E. coli,</i> Enhances Bacterial Pathogenicity by Promoting Adhesion to Epithelial Cells and Survival Within Macrophages."],"pmcid":["PMC12839154"],"funding_grant_id":["31570124","YKK24268","202305041"],"pubmed_authors":["Wu M","Shi R","Chen L","Chen W","Zhang R","Xu Z","Cui A","Qin C","Gao D"],"additional_accession":[]},"is_claimable":false,"name":"EruA, a Regulator of Adherent-Invasive <i>E. coli,</i> Enhances Bacterial Pathogenicity by Promoting Adhesion to Epithelial Cells and Survival Within Macrophages.","description":"Adherent-invasive <i>E. coli</i> (AIEC) is closely related to inflammatory bowel disease (IBD). However, its pathogenic mechanism has not yet been fully elucidated. Using a BLASTP search, we discovered that the amino acid sequence of a putative protein (UFP37798.1) in the AIEC LF82 strain is highly homologous to some regulators in the SlyA family. We named it EruA. We displayed the secondary structures of EruA using bioinformatics, overexpressed the His<sub>6</sub>-tagged EruA protein using SDS-PAGE, and dissected the genetic organization of the <i>eruA</i> chromosomal region using 5'RACE. We constructed an <i>eruA</i> deletion mutant (ΔeruA) and a complementary strain (CΔeruA) of the LF82 strain. The transcriptomes of wild-type (WT) and ΔeruA bacteria were compared using RNA sequencing an","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-09T03:17:48.885Z","creation":"2026-06-09T03:08:21.888Z"},"accession":"S-EPMC12839154","cross_references":{"pubmed":["41594692"],"doi":["10.3390/biom16010152"]}}