{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Trukhin D"],"funding":["Ministry of Science and Higher Education of the Russian Federation"],"pagination":["22"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12839167"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(1)"],"pubmed_abstract":["The detailed characterization of antigen-specific serum antibodies is hindered by the lack of efficient, gentle isolation methods. In this context, standard column affinity chromatography, although a powerful purification tool, presents practical challenges, including high antigen consumption and elution conditions that risk inducing antibody polyreactivity, while conventional acidic elution often compromises antibody integrity. This study introduces a novel microscale method for isolating specific immunoglobulins using anionic detergents as mild eluents. We employed antigen-functionalized hydrogel microarrays and magnetic beads as micro-immunosorbents. Among the tested detergents, sodium lauroyl glutamate (SLG) was optimal, achieving up to 78.3% recovery of functional antibodies. The opti"],"journal":["Biosensors"],"pubmed_title":["Anionic Detergents as Eluents for Microscale Isolation of Antigen-Specific Serum Immunoglobulins."],"pmcid":["PMC12839167"],"funding_grant_id":["075-15-2025-519"],"pubmed_authors":["Gryadunov D","Trukhin D","Filippova M","Savvateeva E","Tskaeva A","Troshina E"],"additional_accession":[]},"is_claimable":false,"name":"Anionic Detergents as Eluents for Microscale Isolation of Antigen-Specific Serum Immunoglobulins.","description":"The detailed characterization of antigen-specific serum antibodies is hindered by the lack of efficient, gentle isolation methods. In this context, standard column affinity chromatography, although a powerful purification tool, presents practical challenges, including high antigen consumption and elution conditions that risk inducing antibody polyreactivity, while conventional acidic elution often compromises antibody integrity. This study introduces a novel microscale method for isolating specific immunoglobulins using anionic detergents as mild eluents. We employed antigen-functionalized hydrogel microarrays and magnetic beads as micro-immunosorbents. Among the tested detergents, sodium lauroyl glutamate (SLG) was optimal, achieving up to 78.3% recovery of functional antibodies. The opti","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-16T03:09:31.806Z","creation":"2026-06-16T03:06:53.363Z"},"accession":"S-EPMC12839167","cross_references":{"pubmed":["41590274"],"doi":["10.3390/bios16010022"]}}