biostudies-literatureTrukhin DMinistry of Science and Higher Education of the Russian Federation22https://www.ebi.ac.uk/biostudies/studies/S-EPMC12839167biostudies-literatureUnknown16(1)The detailed characterization of antigen-specific serum antibodies is hindered by the lack of efficient, gentle isolation methods. In this context, standard column affinity chromatography, although a powerful purification tool, presents practical challenges, including high antigen consumption and elution conditions that risk inducing antibody polyreactivity, while conventional acidic elution often compromises antibody integrity. This study introduces a novel microscale method for isolating specific immunoglobulins using anionic detergents as mild eluents. We employed antigen-functionalized hydrogel microarrays and magnetic beads as micro-immunosorbents. Among the tested detergents, sodium lauroyl glutamate (SLG) was optimal, achieving up to 78.3% recovery of functional antibodies. The optiBiosensorsAnionic Detergents as Eluents for Microscale Isolation of Antigen-Specific Serum Immunoglobulins.PMC12839167075-15-2025-519Gryadunov DTrukhin DFilippova MSavvateeva ETskaeva ATroshina EfalseAnionic Detergents as Eluents for Microscale Isolation of Antigen-Specific Serum Immunoglobulins.The detailed characterization of antigen-specific serum antibodies is hindered by the lack of efficient, gentle isolation methods. In this context, standard column affinity chromatography, although a powerful purification tool, presents practical challenges, including high antigen consumption and elution conditions that risk inducing antibody polyreactivity, while conventional acidic elution often compromises antibody integrity. This study introduces a novel microscale method for isolating specific immunoglobulins using anionic detergents as mild eluents. We employed antigen-functionalized hydrogel microarrays and magnetic beads as micro-immunosorbents. Among the tested detergents, sodium lauroyl glutamate (SLG) was optimal, achieving up to 78.3% recovery of functional antibodies. The opti2025-01-01T00:00:00Z2025 Dec2026-06-16T03:09:31.806Z2026-06-16T03:06:53.363ZS-EPMC128391674159027410.3390/bios16010022