biostudies-literatureWang WNational Natural Science Foundation of ChinaLiang shan Prefecture Science and Technology Bureau266https://www.ebi.ac.uk/biostudies/studies/S-EPMC12840261biostudies-literatureUnknown15(2)Given the potential hazards of enrofloxacin (ENR) residues to human health, establishing an accurate, rapid, and stable detection method is of importance. To enable the direct detection of ENR, an electrochemical sensor was constructed in this study. N- and S-doped carbon quantum dots (CQDs) with peroxidase-like activity were prepared using DL-malic acid, L-alanine, and L-cysteine as precursors and compounded with a tetrakis (4-carboxyphenyl) porphyrin (TCPP) and Fe(NO₃)₃·9H₂O to make novel N/S CQDs@Fe-TCPP composite carbon-based nanozymes to construct an electrochemical sensor, and the electrochemical behavior was investigated. Under optimal experimental conditions, the sensor exhibited a linear current response to ENR concentrations in the range of 1-1300Foods (Basel, Switzerland)Construction of N/S CQDs@Fe-TCPP Nanocatalyst-Induced Electrochemical Sensors for Rapid and Sensitive Detection of Enrofloxacin Residues in Milk.PMC128402613257346723CGZH0006Tang JZou LHong YTang CFu YLiu AWang WHu KHe LLiu SChen SfalseConstruction of N/S CQDs@Fe-TCPP Nanocatalyst-Induced Electrochemical Sensors for Rapid and Sensitive Detection of Enrofloxacin Residues in Milk.Given the potential hazards of enrofloxacin (ENR) residues to human health, establishing an accurate, rapid, and stable detection method is of importance. To enable the direct detection of ENR, an electrochemical sensor was constructed in this study. N- and S-doped carbon quantum dots (CQDs) with peroxidase-like activity were prepared using DL-malic acid, L-alanine, and L-cysteine as precursors and compounded with a tetrakis (4-carboxyphenyl) porphyrin (TCPP) and Fe(NO₃)₃·9H₂O to make novel N/S CQDs@Fe-TCPP composite carbon-based nanozymes to construct an electrochemical sensor, and the electrochemical behavior was investigated. Under optimal experimental conditions, the sensor exhibited a linear current response to ENR concentrations in the range of 1-13002026-01-01T00:00:00Z2026 Jan2026-06-13T03:13:28.808Z2026-06-13T03:08:54.66ZS-EPMC128402614159686510.3390/foods15020266