biostudies-literatureShen Zthe Doctoral New Investigator Grants of Hunan University of Medicinethe Key Scientific Research Project of Hunan Provincial Department of EducationNational Student Innovation and Entrepreneurship Training ProgrammeHunan Provincial Student Innovation and Entrepreneurship Training ProgrammeHunan Provincial Natural Science Foundation Project124https://www.ebi.ac.uk/biostudies/studies/S-EPMC12840552biostudies-literatureUnknown48(1)<h4>Objective</h4>Cerebral ischemia-reperfusion injury (IRI) is a distinct pathological phase that differs from permanent ischemia (IR) in that it triggers secondary damage despite the restoration of blood flow. The primary objective of this study is to comprehensively characterize and compare the molecular signatures-such as differential gene expression, protein activation, and metabolic alterations-between IRI and IR. By doing so, we aim to identify key pathways and biomarkers that specifically drive IRI and IR pathology, thereby providing novel therapeutic targets to mitigate reperfusion-induced damage in stroke and related neurological conditions.<h4>Methods</h4>We employed an integrated transcriptomic and proteomic approach to compare a permanent ischemia model (IR, 24 h ischemia) witCurrent issues in molecular biologyMolecular Dissection of Permanent vs. Reperfused Ischemia: Multi-Omics Divergence and Precision Therapeutic Implications.PMC128405522024JJ7322; 2025JJ704582020122003S202312214012202112214003; 20221221400823A0722Li YShen ZLi JXing XLZhou SJiang HLiu QYang TJing DZhu TLu QfalseMolecular Dissection of Permanent vs. Reperfused Ischemia: Multi-Omics Divergence and Precision Therapeutic Implications.<h4>Objective</h4>Cerebral ischemia-reperfusion injury (IRI) is a distinct pathological phase that differs from permanent ischemia (IR) in that it triggers secondary damage despite the restoration of blood flow. The primary objective of this study is to comprehensively characterize and compare the molecular signatures-such as differential gene expression, protein activation, and metabolic alterations-between IRI and IR. By doing so, we aim to identify key pathways and biomarkers that specifically drive IRI and IR pathology, thereby providing novel therapeutic targets to mitigate reperfusion-induced damage in stroke and related neurological conditions.<h4>Methods</h4>We employed an integrated transcriptomic and proteomic approach to compare a permanent ischemia model (IR, 24 h ischemia) wit2026-01-01T00:00:00Z2026 Jan2026-06-13T03:13:38.727Z2026-06-13T03:08:52.902ZS-EPMC128405524161495410.3390/cimb48010124