{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao C"],"funding":["the Excellent Youth Science Foundation of Heilongjiang Province","the National Key Research and Development Program of China","National Natural Science Foundation of China","the China Postdoctoral Science Foundation"],"pagination":["771"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12840808"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(2)"],"pubmed_abstract":["<i>Fragaria vesca</i> L., a widely distributed model species, serves as a key resource for studying the evolution and genetics of the Fragaria genus. Research has shown that R2R3-MYB transcription factors are crucial for plant growth and development. However, their specific role in cold resistance in <i>F. vesca</i> is not well understood. In this study, we used the latest genome data for the strawberry (<i>F. vesca</i> v6.0). We performed a genome-wide identification of the R2R3-MYB gene family in <i>F. vesca</i>. We identified a total of 106 R2R3-FvMYBs. Based on their predicted functions in plants, we classified these genes into 25 distinct subfamilies. We then conducted a comprehensive bioinformatics analysis of this family. We performed a detailed examination of the R2R3-FvMYBs structures and physicochemical properties. This analysis provided five key parameters for each protein: molecular weight, the number of amino acids, theoretical isoelectric point, grand average of hydropathicity (GRAVY), and instability index. Gene duplication analysis suggested that segmental duplications were a primary driver of the proliferation of this gene family. Promoter cis-acting element prediction revealed that a large proportion of R2R3-FvMYBs possess elements predominantly associated with phytohormone responsiveness and biotic/abiotic stress responses. Quantitative real-time reverse transcription PCR (qRT-PCR) results confirmed that the expression levels of several R2R3-FvMYBs were upregulated under cold stress. Furthermore, compared to wild-type controls, the overexpression of <i>FvMYB103</i> in <i>Arabidopsis thaliana</i> enhanced cold tolerance, accompanied by increases in the relevant physiological indices. Collectively, these findings support further investigation into R2R3-MYB gene family to directly assess their contribution to cold resistance."],"journal":["International journal of molecular sciences"],"pubmed_title":["Genome-Wide Identification of R2R3-MYB Gene Family in Strawberry (<i>Fragaria vesca</i> L.) and Functional Characterization of <i>FvMYB103</i> in Cold Stress."],"pmcid":["PMC12840808"],"funding_grant_id":["2023MD744175","2022YFD1600501-13","32172521","32402458","YQ2023C006"],"pubmed_authors":["Li W","Huang F","Li X","Zhao C","Chen Z","Chen X","Zhang L","Wang Y","Han D","Liu W"],"additional_accession":[]},"is_claimable":false,"name":"Genome-Wide Identification of R2R3-MYB Gene Family in Strawberry (<i>Fragaria vesca</i> L.) and Functional Characterization of <i>FvMYB103</i> in Cold Stress.","description":"<i>Fragaria vesca</i> L., a widely distributed model species, serves as a key resource for studying the evolution and genetics of the Fragaria genus. Research has shown that R2R3-MYB transcription factors are crucial for plant growth and development. However, their specific role in cold resistance in <i>F. vesca</i> is not well understood. In this study, we used the latest genome data for the strawberry (<i>F. vesca</i> v6.0). We performed a genome-wide identification of the R2R3-MYB gene family in <i>F. vesca</i>. We identified a total of 106 R2R3-FvMYBs. Based on their predicted functions in plants, we classified these genes into 25 distinct subfamilies. We then conducted a comprehensive bioinformatics analysis of this family. We performed a detailed examination of the R2R3-FvMYBs structures and physicochemical properties. This analysis provided five key parameters for each protein: molecular weight, the number of amino acids, theoretical isoelectric point, grand average of hydropathicity (GRAVY), and instability index. Gene duplication analysis suggested that segmental duplications were a primary driver of the proliferation of this gene family. Promoter cis-acting element prediction revealed that a large proportion of R2R3-FvMYBs possess elements predominantly associated with phytohormone responsiveness and biotic/abiotic stress responses. Quantitative real-time reverse transcription PCR (qRT-PCR) results confirmed that the expression levels of several R2R3-FvMYBs were upregulated under cold stress. Furthermore, compared to wild-type controls, the overexpression of <i>FvMYB103</i> in <i>Arabidopsis thaliana</i> enhanced cold tolerance, accompanied by increases in the relevant physiological indices. Collectively, these findings support further investigation into R2R3-MYB gene family to directly assess their contribution to cold resistance.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-15T03:17:00.091Z","creation":"2026-06-15T03:10:54.286Z"},"accession":"S-EPMC12840808","cross_references":{"pubmed":["41596420"],"doi":["10.3390/ijms27020771"]}}