biostudies-literatureZhao Cthe Excellent Youth Science Foundation of Heilongjiang Provincethe National Key Research and Development Program of ChinaNational Natural Science Foundation of Chinathe China Postdoctoral Science Foundation771https://www.ebi.ac.uk/biostudies/studies/S-EPMC12840808biostudies-literatureUnknown27(2)<i>Fragaria vesca</i> L., a widely distributed model species, serves as a key resource for studying the evolution and genetics of the Fragaria genus. Research has shown that R2R3-MYB transcription factors are crucial for plant growth and development. However, their specific role in cold resistance in <i>F. vesca</i> is not well understood. In this study, we used the latest genome data for the strawberry (<i>F. vesca</i> v6.0). We performed a genome-wide identification of the R2R3-MYB gene family in <i>F. vesca</i>. We identified a total of 106 R2R3-FvMYBs. Based on their predicted functions in plants, we classified these genes into 25 distinct subfamilies. We then conducted a comprehensive bioinformatics analysis of this family. We performed a detailed examination of the R2R3-FvMYBs structures and physicochemical properties. This analysis provided five key parameters for each protein: molecular weight, the number of amino acids, theoretical isoelectric point, grand average of hydropathicity (GRAVY), and instability index. Gene duplication analysis suggested that segmental duplications were a primary driver of the proliferation of this gene family. Promoter cis-acting element prediction revealed that a large proportion of R2R3-FvMYBs possess elements predominantly associated with phytohormone responsiveness and biotic/abiotic stress responses. Quantitative real-time reverse transcription PCR (qRT-PCR) results confirmed that the expression levels of several R2R3-FvMYBs were upregulated under cold stress. Furthermore, compared to wild-type controls, the overexpression of <i>FvMYB103</i> in <i>Arabidopsis thaliana</i> enhanced cold tolerance, accompanied by increases in the relevant physiological indices. Collectively, these findings support further investigation into R2R3-MYB gene family to directly assess their contribution to cold resistance.International journal of molecular sciencesGenome-Wide Identification of R2R3-MYB Gene Family in Strawberry (<i>Fragaria vesca</i> L.) and Functional Characterization of <i>FvMYB103</i> in Cold Stress.PMC128408082023MD7441752022YFD1600501-133217252132402458YQ2023C006Li WHuang FLi XZhao CChen ZChen XZhang LWang YHan DLiu WfalseGenome-Wide Identification of R2R3-MYB Gene Family in Strawberry (<i>Fragaria vesca</i> L.) and Functional Characterization of <i>FvMYB103</i> in Cold Stress.<i>Fragaria vesca</i> L., a widely distributed model species, serves as a key resource for studying the evolution and genetics of the Fragaria genus. Research has shown that R2R3-MYB transcription factors are crucial for plant growth and development. However, their specific role in cold resistance in <i>F. vesca</i> is not well understood. In this study, we used the latest genome data for the strawberry (<i>F. vesca</i> v6.0). We performed a genome-wide identification of the R2R3-MYB gene family in <i>F. vesca</i>. We identified a total of 106 R2R3-FvMYBs. Based on their predicted functions in plants, we classified these genes into 25 distinct subfamilies. We then conducted a comprehensive bioinformatics analysis of this family. We performed a detailed examination of the R2R3-FvMYBs structures and physicochemical properties. This analysis provided five key parameters for each protein: molecular weight, the number of amino acids, theoretical isoelectric point, grand average of hydropathicity (GRAVY), and instability index. Gene duplication analysis suggested that segmental duplications were a primary driver of the proliferation of this gene family. Promoter cis-acting element prediction revealed that a large proportion of R2R3-FvMYBs possess elements predominantly associated with phytohormone responsiveness and biotic/abiotic stress responses. Quantitative real-time reverse transcription PCR (qRT-PCR) results confirmed that the expression levels of several R2R3-FvMYBs were upregulated under cold stress. Furthermore, compared to wild-type controls, the overexpression of <i>FvMYB103</i> in <i>Arabidopsis thaliana</i> enhanced cold tolerance, accompanied by increases in the relevant physiological indices. Collectively, these findings support further investigation into R2R3-MYB gene family to directly assess their contribution to cold resistance.2026-01-01T00:00:00Z2026 Jan2026-06-15T03:17:00.091Z2026-06-15T03:10:54.286ZS-EPMC128408084159642010.3390/ijms27020771