{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhao SH"],"funding":["Basic Research Funds of Hebei Academy of Agriculture and Forestry Sciences","Hebei Agriculture Research System"],"pagination":["57"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12842091"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(1)"],"pubmed_abstract":["The polyphagous pest <i>Oxycetonia jucunda</i> Faldermann can cause substantial damage to a range of economically important crops, with the adult beetles feeding directly on floral tissues and young leaves. RT-qPCR is widely used to analyze gene expression, for which the selection of stable reference genes is essential for enabling an accurate normalization of expression. However, no systematic evaluations of suitable reference genes for RT-qPCR analysis using different tissues of <i>O. jucunda</i> have been conducted. To assess their applicability as reliable normalization controls, we used five computational methods to examine the stability of seven potential reference genes (<i>GAPDH</i>, <i>EF1α</i>, <i>RPS3</i>, <i>RPS18</i>, <i>RPL18</i>, <i>RPS31</i>, and <i>UBC5A</i>) across six adult tissues, with three biological replicates per tissue. The findings revealed <i>RPS3</i> and <i>RPS31</i> to be the most stably expressed. This pair of reference genes was further validated by normalizing the expression of the odorant-binding protein 3 (<i>OBP3</i>) target gene. Our findings will provide important foundational data for the accurate analysis of functional gene expression in <i>O. jucunda</i>."],"journal":["Insects"],"pubmed_title":["Validation of Stable Reference Genes for RT-qPCR Normalization in <i>Oxycetonia jucunda</i> (Coleoptera: Scarabaeidae)."],"pmcid":["PMC12842091"],"funding_grant_id":["HBCT2024150208","2025100102"],"pubmed_authors":["Gao Q","Zhou N","Zhang SP","Xu GL","Zhao JQ","Zhao SH","Yu RT","Yue Y"],"additional_accession":[]},"is_claimable":false,"name":"Validation of Stable Reference Genes for RT-qPCR Normalization in <i>Oxycetonia jucunda</i> (Coleoptera: Scarabaeidae).","description":"The polyphagous pest <i>Oxycetonia jucunda</i> Faldermann can cause substantial damage to a range of economically important crops, with the adult beetles feeding directly on floral tissues and young leaves. RT-qPCR is widely used to analyze gene expression, for which the selection of stable reference genes is essential for enabling an accurate normalization of expression. However, no systematic evaluations of suitable reference genes for RT-qPCR analysis using different tissues of <i>O. jucunda</i> have been conducted. To assess their applicability as reliable normalization controls, we used five computational methods to examine the stability of seven potential reference genes (<i>GAPDH</i>, <i>EF1α</i>, <i>RPS3</i>, <i>RPS18</i>, <i>RPL18</i>, <i>RPS31</i>, and <i>UBC5A</i>) across six adult tissues, with three biological replicates per tissue. The findings revealed <i>RPS3</i> and <i>RPS31</i> to be the most stably expressed. This pair of reference genes was further validated by normalizing the expression of the odorant-binding protein 3 (<i>OBP3</i>) target gene. Our findings will provide important foundational data for the accurate analysis of functional gene expression in <i>O. jucunda</i>.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-18T03:09:17.767Z","creation":"2026-06-18T03:07:30.374Z"},"accession":"S-EPMC12842091","cross_references":{"pubmed":["41598911"],"doi":["10.3390/insects17010057"]}}