biostudies-literatureGonzalez-Iglesias DAxencia Galega de InnovacionMinisterio de Ciencia, Innovación y Universidades277https://www.ebi.ac.uk/biostudies/studies/S-EPMC12843702biostudies-literatureUnknown31(2)An environmentally friendly extraction strategy based on an MSAT (Medium Scale Ambient Temperature) system was applied to <i>Quercus ilex</i> and <i>Quercus robur</i> acorns with the aim of maximizing polyphenolic yield and antioxidant activity while minimizing solvent consumption. Operational parameters were first optimized for <i>Quercus ilex</i> using a BBD-RSM (Box-Behnken response surface methodology), where the optimum working zone corresponds to the values of 200 g of acorn, 100 mL of extracting solvent, and 0.5 dispersant/acorn ratio. Subsequently, these conditions were applied to <i>Quercus robur</i> to enable an interspecific comparison. Extracts were evaluated in terms of total polyphenolic content, antioxidant activity, reducing sugars, proteins, targeted polyphenols quantifiedMolecules (Basel, Switzerland)Comparative Analysis of Phytochemical Profile and Antioxidant and Antimicrobial Activity of Green Extracts from <i>Quercus ilex</i> and <i>Quercus robur</i> Acorns.PMC1284370201_IN606D_2022_2665353PTQ2022-012516Vielba JMLores MMiguel TGonzalez-Iglesias DMartinez-Vazquez FRubio LfalseComparative Analysis of Phytochemical Profile and Antioxidant and Antimicrobial Activity of Green Extracts from <i>Quercus ilex</i> and <i>Quercus robur</i> Acorns.An environmentally friendly extraction strategy based on an MSAT (Medium Scale Ambient Temperature) system was applied to <i>Quercus ilex</i> and <i>Quercus robur</i> acorns with the aim of maximizing polyphenolic yield and antioxidant activity while minimizing solvent consumption. Operational parameters were first optimized for <i>Quercus ilex</i> using a BBD-RSM (Box-Behnken response surface methodology), where the optimum working zone corresponds to the values of 200 g of acorn, 100 mL of extracting solvent, and 0.5 dispersant/acorn ratio. Subsequently, these conditions were applied to <i>Quercus robur</i> to enable an interspecific comparison. Extracts were evaluated in terms of total polyphenolic content, antioxidant activity, reducing sugars, proteins, targeted polyphenols quantified2026-01-01T00:00:00Z2026 Jan2026-06-10T03:17:04.952Z2026-06-10T03:12:44.18ZS-EPMC128437024159932610.3390/molecules31020277