{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Neri I"],"funding":["Edinburgh Napier University"],"pagination":["379"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12843750"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(2)"],"pubmed_abstract":["A comprehensive two-dimensional liquid chromatography platform (LC × LC) was developed and validated for dermal permeability studies. In this implementation, the two separation dimensions were applied to mimic the layered structure of human skin: a ceramide-like stationary phase in the first dimension (<sup>1</sup>D) to simulate the lipid-rich epidermis, and an immobilized artificial membrane (IAM) phase in the second (<sup>2</sup>D) to emulate the dermis. Experimental conditions were optimised to reflect the microenvironment of the in vivo skin. For validation purposes, 43 pharmaceutical and cosmetic compounds whose transdermal permeability coefficients (log K<sub>p</sub>) were known from the scientific literature were selected as model solutes. A good degree of separation was achieved ac"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Integrated Biomimetic 2D-LC and Permeapad<sup>®</sup> Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds."],"pmcid":["PMC12843750"],"funding_grant_id":["n.a."],"pubmed_authors":["Grumetto L","Russo G","Stevens C","Neri I"],"additional_accession":[]},"is_claimable":false,"name":"Integrated Biomimetic 2D-LC and Permeapad<sup>®</sup> Assay for Profiling the Transdermal Diffusion of Pharmaceutical Compounds.","description":"A comprehensive two-dimensional liquid chromatography platform (LC × LC) was developed and validated for dermal permeability studies. In this implementation, the two separation dimensions were applied to mimic the layered structure of human skin: a ceramide-like stationary phase in the first dimension (<sup>1</sup>D) to simulate the lipid-rich epidermis, and an immobilized artificial membrane (IAM) phase in the second (<sup>2</sup>D) to emulate the dermis. Experimental conditions were optimised to reflect the microenvironment of the in vivo skin. For validation purposes, 43 pharmaceutical and cosmetic compounds whose transdermal permeability coefficients (log K<sub>p</sub>) were known from the scientific literature were selected as model solutes. A good degree of separation was achieved ac","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-15T03:14:48.759Z","creation":"2026-06-15T03:08:45.508Z"},"accession":"S-EPMC12843750","cross_references":{"pubmed":["41599428"],"doi":["10.3390/molecules31020379"]}}