{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang X"],"funding":["National Natural Science Foundation of China","the National Key R&D Program of China"],"pagination":["285"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12844060"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(2)"],"pubmed_abstract":["<i>Portunus trituberculatus</i> is an economically important marine crustacean in East Asia's aquaculture industry. Nevertheless, precise genome modification has not yet been established. In this study, we evaluated the applicability of the CRISPR-Cas9 gene editing system in <i>P. trituberculatus</i> using electroporation for efficient delivery of the Cas9-sgRNA complex into zygotes. We systematically investigated electroporation parameters, including buffer composition, voltage, capacitance, and pulse times. Our results showed that artificial seawater was a superior buffer to phosphate-buffered saline (PBS) and identified an effective electroporation condition of 600 V, 1 μF capacitance, and two pulses, resulting in approximately 72.7% fluorescent zygotes. Under these electroporated condi"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Establishment of CRISPR-Cas9-Mediated Gene Editing in the Swimming Crab <i>Portunus trituberculatus</i>."],"pmcid":["PMC12844060"],"funding_grant_id":["2022YFD2400104","32341058 and 31972783"],"pubmed_authors":["Liu L","Shi C","Wang C","Wang X","Chen X","Li R","Zhou Y","Song W","Zhao Y","Mu C"],"additional_accession":[]},"is_claimable":false,"name":"Establishment of CRISPR-Cas9-Mediated Gene Editing in the Swimming Crab <i>Portunus trituberculatus</i>.","description":"<i>Portunus trituberculatus</i> is an economically important marine crustacean in East Asia's aquaculture industry. Nevertheless, precise genome modification has not yet been established. In this study, we evaluated the applicability of the CRISPR-Cas9 gene editing system in <i>P. trituberculatus</i> using electroporation for efficient delivery of the Cas9-sgRNA complex into zygotes. We systematically investigated electroporation parameters, including buffer composition, voltage, capacitance, and pulse times. Our results showed that artificial seawater was a superior buffer to phosphate-buffered saline (PBS) and identified an effective electroporation condition of 600 V, 1 μF capacitance, and two pulses, resulting in approximately 72.7% fluorescent zygotes. Under these electroporated condi","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-10T03:17:55.591Z","creation":"2026-06-10T03:12:52.2Z"},"accession":"S-EPMC12844060","cross_references":{"pubmed":["41599334"],"doi":["10.3390/molecules31020285"]}}