{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Ortega C"],"pubmed_abstract":["Rubella virus (RV) IgG quantification is essential for verifying immunity, particularly in prenatal care. However, substantial variability exists among commercial immunoassays, especially when testing low-antibody sera. In this study, we evaluated five commercial assays-four chemiluminescent immunoassays (CLIAs) and one Enzyme-linked Immunosorbent Assay (ELISA)-using a recombinant immunoblot (IB) as the reference method. A panel of 137 serum samples with low or undetectable IgG levels was analyzed. Sensitivity ranged from 19.6% to 70.1%, while specificity exceeded 94%. Only 18.6% of immunoblot-positive samples tested positive across all assays. Marked quantitative differences were observed, with the Atellica assay yielding the highest titers and Alinity the lowest. Reclassifying equivocal "],"journal":["Microorganisms"],"pagination":["58"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12844522"],"repository":["biostudies-literature"],"pubmed_title":["Evaluation of Commercial Immunoassays for Rubella Virus IgG Detection in Low-Antibody Sera Using a Recombinant Immunoblot as a Reference Method."],"pmcid":["PMC12844522"],"pubmed_authors":["Reguera JA","Rodriguez-Granger J","Cobo F","Abreu A","Ortega C","Sampedro-Padilla A","Sampedro A","Serrano J","Gutierrez-Bautista JF","Mazuelas P"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of Commercial Immunoassays for Rubella Virus IgG Detection in Low-Antibody Sera Using a Recombinant Immunoblot as a Reference Method.","description":"Rubella virus (RV) IgG quantification is essential for verifying immunity, particularly in prenatal care. However, substantial variability exists among commercial immunoassays, especially when testing low-antibody sera. In this study, we evaluated five commercial assays-four chemiluminescent immunoassays (CLIAs) and one Enzyme-linked Immunosorbent Assay (ELISA)-using a recombinant immunoblot (IB) as the reference method. A panel of 137 serum samples with low or undetectable IgG levels was analyzed. Sensitivity ranged from 19.6% to 70.1%, while specificity exceeded 94%. Only 18.6% of immunoblot-positive samples tested positive across all assays. Marked quantitative differences were observed, with the Atellica assay yielding the highest titers and Alinity the lowest. Reclassifying equivocal ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-15T03:19:59.108Z","creation":"2026-06-15T03:11:21.521Z"},"accession":"S-EPMC12844522","cross_references":{"pubmed":["41597578"],"doi":["10.3390/microorganisms14010058"]}}