{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Camporesi G"],"funding":["Italian Ministry of University and Research","Ministero dell''Università e della Ricerca"],"pagination":["339"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12844764"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(2)"],"pubmed_abstract":["<b>Background/Objectives</b>: Assessing the bioavailability of nutrients and bioactive compounds in vitro commonly relies on coupling standardized gastrointestinal digestion models with intestinal epithelial cell systems. However, digests produced using static digestion protocols such as INFOGEST often impair epithelial barrier integrity, limiting their direct application to intestinal models and reducing reproducibility across studies. <b>Methods</b>: This work systematically compared five commonly used digest conditioning strategies, including acidification, centrifugation, rapid freezing, and ultrafiltration using 10 kDa and 3 kDa molecular weight cut-off membranes, to identify the approach that best preserves intestinal epithelial viability and barrier function while enabling exposure "],"journal":["Nutrients"],"pubmed_title":["Optimizing INFOGEST Digest Conditioning for Reliable In Vitro Assessment of Nutrient Bioavailability Using Caco-2 Cell Models."],"pmcid":["PMC12844764"],"funding_grant_id":["PE00000003"],"pubmed_authors":["Canzian C","Camporesi G","Bordoni A"],"additional_accession":[]},"is_claimable":false,"name":"Optimizing INFOGEST Digest Conditioning for Reliable In Vitro Assessment of Nutrient Bioavailability Using Caco-2 Cell Models.","description":"<b>Background/Objectives</b>: Assessing the bioavailability of nutrients and bioactive compounds in vitro commonly relies on coupling standardized gastrointestinal digestion models with intestinal epithelial cell systems. However, digests produced using static digestion protocols such as INFOGEST often impair epithelial barrier integrity, limiting their direct application to intestinal models and reducing reproducibility across studies. <b>Methods</b>: This work systematically compared five commonly used digest conditioning strategies, including acidification, centrifugation, rapid freezing, and ultrafiltration using 10 kDa and 3 kDa molecular weight cut-off membranes, to identify the approach that best preserves intestinal epithelial viability and barrier function while enabling exposure ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-13T03:13:02.905Z","creation":"2026-06-13T03:08:55.379Z"},"accession":"S-EPMC12844764","cross_references":{"pubmed":["41599952"],"doi":["10.3390/nu18020339"]}}