{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nemec KM"],"funding":["NIA NIH HHS","NIH Office of the Director","NIAID NIH HHS","NIMH NIH HHS","The Paul Allen Frontiers Group","Blavatnik Family Foundation","National Institute of Allergy and Infectious Diseases","National Institute of Health","NINDS NIH HHS","NIGMS NIH HHS","NIH HHS","National Science Foundation","Esther A. and Joseph Klingenstein Fund"],"pagination":["RP102900"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12844908"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14"],"pubmed_abstract":["Microglia, the brain's resident macrophages, can be reconstituted by surrogate cells - a process termed 'microglia replacement'. To expand the microglia replacement toolkit, we here introduce estrogen-regulated (ER) homeobox B8 (Hoxb8) conditionally immortalized macrophages, a cell model for generation of immune cells from murine bone marrow, as a versatile model for microglia replacement. We find that ER-Hoxb8 macrophages are highly comparable to primary bone marrow-derived macrophages in vitro, and, when transplanted into a microglia-free brain, engraft the parenchyma and differentiate into microglia-like cells. Furthermore, ER-Hoxb8 progenitors are readily transducible by virus and easily stored as stable, genetically manipulated cell lines. As a demonstration of this system's power for"],"journal":["eLife"],"pubmed_title":["Microglia replacement by ER-Hoxb8 conditionally immortalized macrophages provides insight into Aicardi-Goutieres syndrome neuropathology."],"pmcid":["PMC12844908"],"funding_grant_id":["R01AI139544","Blavatnik Family Fellowship","NIH T32 GM008076","DGE-1845298","GRT-00000774","R01NS120960","R15 NS133939","Fellowship Award in Neuroscience","R01 AI139544","R01 NS120960","T32-AG-000255-26","T32 GM008076","DP5 OD036159","DP5OD036159","T32MH019112","T32 MH019112","R15NS133939","R35GM138085","R01 NS134651","R35 GM138085","R01NS134651","T32 GM007170","T32 AG000255"],"pubmed_authors":["Nemec KM","Uy G","Purnell FS","Thaiss CA","Guo X","Oon CH","O'Reilly ML","O'Brien CA","Elfayoumi B","Lombroso SI","Byerly L","Chaluvadi VS","Williamson AP","Bennett ML","Blank N","Rawat P","Wang Q","Bennett FC","Yaqoob F","Bailis W","Aisenberg WH","Temsamrit B"],"additional_accession":[]},"is_claimable":false,"name":"Microglia replacement by ER-Hoxb8 conditionally immortalized macrophages provides insight into Aicardi-Goutieres syndrome neuropathology.","description":"Microglia, the brain's resident macrophages, can be reconstituted by surrogate cells - a process termed 'microglia replacement'. To expand the microglia replacement toolkit, we here introduce estrogen-regulated (ER) homeobox B8 (Hoxb8) conditionally immortalized macrophages, a cell model for generation of immune cells from murine bone marrow, as a versatile model for microglia replacement. We find that ER-Hoxb8 macrophages are highly comparable to primary bone marrow-derived macrophages in vitro, and, when transplanted into a microglia-free brain, engraft the parenchyma and differentiate into microglia-like cells. Furthermore, ER-Hoxb8 progenitors are readily transducible by virus and easily stored as stable, genetically manipulated cell lines. As a demonstration of this system's power for","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-14T21:03:49.846Z","creation":"2026-06-25T03:08:16.632Z"},"accession":"S-EPMC12844908","cross_references":{"pubmed":["41589671"],"doi":["10.7554/eLife.102900"]}}