biostudies-literatureCarini VLiverpool John Moores University13https://www.ebi.ac.uk/biostudies/studies/S-EPMC12845076biostudies-literatureUnknown18(1)<b>Background/Objectives:</b> The growing threat posed by antimicrobial resistance to worldwide public health highlights the urgent need not only for new anti-infective candidates, but also for innovative formulation strategies capable of mediating effective delivery of anti-infective compounds. The current study, therefore, aimed to demonstrate the feasibility of formulating lipid-polymer hybrid nanoparticles (LPHNPs) with dual loading of both core and shell compartments for combination anti-infective delivery. <b>Methods:</b> LPHNPs containing the antibiotic cefotaxime within a chitosan polymer core and the novel antimicrobial peptide RN7IN6 within a bacteria-mimicking lipid shell were produced by microfluidic mixing, and optimized with respect to parameters including total flow rate, flPharmaceuticsDual Core-Shell Loaded Lipid-Polymer Hybrid Nanoparticles as Combination Anti-Infective Delivery Platforms.PMC12845076SCS ECR PhD StudentshipScagnetti GSaleem IFoulkes JEvans KCarini VGordon SfalseDual Core-Shell Loaded Lipid-Polymer Hybrid Nanoparticles as Combination Anti-Infective Delivery Platforms.<b>Background/Objectives:</b> The growing threat posed by antimicrobial resistance to worldwide public health highlights the urgent need not only for new anti-infective candidates, but also for innovative formulation strategies capable of mediating effective delivery of anti-infective compounds. The current study, therefore, aimed to demonstrate the feasibility of formulating lipid-polymer hybrid nanoparticles (LPHNPs) with dual loading of both core and shell compartments for combination anti-infective delivery. <b>Methods:</b> LPHNPs containing the antibiotic cefotaxime within a chitosan polymer core and the novel antimicrobial peptide RN7IN6 within a bacteria-mimicking lipid shell were produced by microfluidic mixing, and optimized with respect to parameters including total flow rate, fl2025-01-01T00:00:00Z2025 Dec2026-06-15T03:12:15.905Z2026-06-15T03:08:38.196ZS-EPMC128450764159912010.3390/pharmaceutics18010013