biostudies-literatureNguyen-Edquilang JU.S. National Science Foundation209https://www.ebi.ac.uk/biostudies/studies/S-EPMC12845159biostudies-literatureUnknown15(2)Papaya (<i>Carica papaya</i> L.) is a tropical trioecious crop with males, hermaphrodites, and females. There is a sequence difference between male and hermaphrodite <i>SHORT VEGETATIVE PHASE</i> (<i>CpSVP</i>), making <i>SVP</i> a strong candidate gene controlling peduncle length in papaya. To study the spatial and temporal expression and function of <i>CpSVP</i> in <i>Arabidopsis</i>, we constructed a translation fusion structure based on the native promoter of <i>SVP</i> in papaya. In the 2kb promoter, strong GUS staining was observed in the floral organs and pedicels. In the 1kb promoter, there is no GUS expression in the floral organs, and it is barely detectable in pedicels. Removal of a GA responsive P-box <i>cis</i>-element in the 1kb promoter enhanced expression in the floral orgaPlants (Basel, Switzerland)Identification of a GA-Related <i>Cis</i>-Element Regulating Male Peduncle Elongation in Papaya.PMC12845159DBI-1546890Ming RNguyen-Edquilang JYue JfalseIdentification of a GA-Related <i>Cis</i>-Element Regulating Male Peduncle Elongation in Papaya.Papaya (<i>Carica papaya</i> L.) is a tropical trioecious crop with males, hermaphrodites, and females. There is a sequence difference between male and hermaphrodite <i>SHORT VEGETATIVE PHASE</i> (<i>CpSVP</i>), making <i>SVP</i> a strong candidate gene controlling peduncle length in papaya. To study the spatial and temporal expression and function of <i>CpSVP</i> in <i>Arabidopsis</i>, we constructed a translation fusion structure based on the native promoter of <i>SVP</i> in papaya. In the 2kb promoter, strong GUS staining was observed in the floral organs and pedicels. In the 1kb promoter, there is no GUS expression in the floral organs, and it is barely detectable in pedicels. Removal of a GA responsive P-box <i>cis</i>-element in the 1kb promoter enhanced expression in the floral orga2026-01-01T00:00:00Z2026 Jan2026-06-16T03:20:03.728Z2026-06-16T03:10:04.654ZS-EPMC128451594160001510.3390/plants15020209