{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(7)"],"submitter":["Sanchez-Pupo RE"],"pubmed_abstract":["<b>Rationale:</b> Chimeric antigen receptor (CAR) T cell therapies have shown remarkable success in treating hematological cancers and are increasingly demonstrating potential for solid tumors. CRISPR-based genome editing offers a promising approach to enhance CAR-T cell potency and safety, yet challenges such as inefficient tumor homing and toxicities in normal tissues, limit broader adoption. Advanced imaging technologies, including bioluminescence imaging (BLI) and positron emission tomography (PET), provide real-time visualization of CAR-T cell behavior <i>in vivo</i>. Here, we developed <i>Trackable Reporter Adaptable CRISPR-Edited CAR</i> (tRACE-CAR) T cells, a modular system for site-specific integration of CARs and imaging reporters. <b>Methods:</b> The luciferase reporter AkaLucif"],"journal":["Theranostics"],"pagination":["3227-3245"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12846014"],"repository":["biostudies-literature"],"pubmed_title":["Imaging CRISPR-edited CAR-T cell therapies with optical and positron emission tomography reporters."],"pmcid":["PMC12846014"],"pubmed_authors":["Xia Y","Shalaby N","Fox MS","Kelly JJ","Martinez-Santiesteban FM","Thiessen JD","Lau J","Hicks JW","Verriet IE","Sanchez-Pupo RE","Ronald JA"],"additional_accession":[]},"is_claimable":false,"name":"Imaging CRISPR-edited CAR-T cell therapies with optical and positron emission tomography reporters.","description":"<b>Rationale:</b> Chimeric antigen receptor (CAR) T cell therapies have shown remarkable success in treating hematological cancers and are increasingly demonstrating potential for solid tumors. CRISPR-based genome editing offers a promising approach to enhance CAR-T cell potency and safety, yet challenges such as inefficient tumor homing and toxicities in normal tissues, limit broader adoption. Advanced imaging technologies, including bioluminescence imaging (BLI) and positron emission tomography (PET), provide real-time visualization of CAR-T cell behavior <i>in vivo</i>. Here, we developed <i>Trackable Reporter Adaptable CRISPR-Edited CAR</i> (tRACE-CAR) T cells, a modular system for site-specific integration of CARs and imaging reporters. <b>Methods:</b> The luciferase reporter AkaLucif","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026","modification":"2026-06-17T07:00:11.229Z","creation":"2026-06-17T03:10:23.325Z"},"accession":"S-EPMC12846014","cross_references":{"pubmed":["41608579"],"doi":["10.7150/thno.119013"]}}