biostudies-literatureUnknown15Li JT<h4>Background</h4>Glioblastoma (GBM) is the most aggressive and prevalent malignant brain tumor in adults, with poor prognosis despite current therapies. Cyclin-dependent kinase 1 (CDK1), a master regulator of cell cycle progression, has been implicated in oncogenesis, but its downstream phosphorylation network in GBM remains incompletely defined.<h4>Methods</h4>CDK1 expression was examined in clinical GBM tissues and cell lines. Functional studies were performed in U251 cells using CDK1-specific shRNAs. Label-free phosphoproteomic profiling and bioinformatics analyses were conducted to map CDK1-regulated signaling pathways and substrates. Prognostic associations were evaluated using Clinical Proteomic Tumor Analysis Consortium (CPTAC) datasets, and functional assays were used to validateFrontiers in oncology1646698https://www.ebi.ac.uk/biostudies/studies/S-EPMC12846947biostudies-literatureCDK1-driven phosphorylation networks promote glioblastoma progression via MAP1B-mediated microtubule destabilization.PMC12846947Li JTLi MDMa CXGuo YJQian RJLi ZXfalseCDK1-driven phosphorylation networks promote glioblastoma progression via MAP1B-mediated microtubule destabilization.<h4>Background</h4>Glioblastoma (GBM) is the most aggressive and prevalent malignant brain tumor in adults, with poor prognosis despite current therapies. Cyclin-dependent kinase 1 (CDK1), a master regulator of cell cycle progression, has been implicated in oncogenesis, but its downstream phosphorylation network in GBM remains incompletely defined.<h4>Methods</h4>CDK1 expression was examined in clinical GBM tissues and cell lines. Functional studies were performed in U251 cells using CDK1-specific shRNAs. Label-free phosphoproteomic profiling and bioinformatics analyses were conducted to map CDK1-regulated signaling pathways and substrates. Prognostic associations were evaluated using Clinical Proteomic Tumor Analysis Consortium (CPTAC) datasets, and functional assays were used to validate2025-01-01T00:00:00Z20252026-06-12T03:19:04.953Z2026-06-12T03:12:00.226ZS-EPMC128469474161354210.3389/fonc.2025.1646698