{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Hu Z"],"pubmed_abstract":["<h4>Introduction</h4>The rising prevalence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) underscores an urgent need for novel antibacterial strategies. Although the natural antibacterial agent berberine sulfate exhibits inhibitory effects against MRSA, its precise molecular targets and mechanisms of action remain unclear.<h4>Methods</h4>To elucidate its mechanism, this study employed quantitative proteomics to analyze protein expression changes in MRSA before and after drug treatment. Acetylomics was further applied to investigate the impact on post-translational modifications. Key findings were functionally validated using site-directed mutagenesis and electrophoretic mobility shift assays (EMSA).<h4>Results</h4>Quantitative proteomics identified 255 differentially expresse"],"journal":["Frontiers in microbiology"],"pagination":["1732962"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12847299"],"repository":["biostudies-literature"],"pubmed_title":["Proteomics and acetyl-proteomics reveal the antibacterial mechanism of berberine sulfate against methicillin-resistant <i>Staphylococcus aureus</i>."],"pmcid":["PMC12847299"],"pubmed_authors":["Liu H","Tan M","Lin C","Hu Z","Chen M","Bian Y","Liu S","Sun Y","Zhou J","Xu JY"],"additional_accession":[]},"is_claimable":false,"name":"Proteomics and acetyl-proteomics reveal the antibacterial mechanism of berberine sulfate against methicillin-resistant <i>Staphylococcus aureus</i>.","description":"<h4>Introduction</h4>The rising prevalence of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) underscores an urgent need for novel antibacterial strategies. Although the natural antibacterial agent berberine sulfate exhibits inhibitory effects against MRSA, its precise molecular targets and mechanisms of action remain unclear.<h4>Methods</h4>To elucidate its mechanism, this study employed quantitative proteomics to analyze protein expression changes in MRSA before and after drug treatment. Acetylomics was further applied to investigate the impact on post-translational modifications. Key findings were functionally validated using site-directed mutagenesis and electrophoretic mobility shift assays (EMSA).<h4>Results</h4>Quantitative proteomics identified 255 differentially expresse","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-10T08:55:23.034Z","creation":"2026-06-10T03:12:37.891Z"},"accession":"S-EPMC12847299","cross_references":{"pubmed":["41614126"],"doi":["10.3389/fmicb.2025.1732962"]}}