{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jassinskaja M"],"funding":["Cancer Research UK","European Research Council","Medical Research Council","Japan Agency for Medical Research and Development","Blood Cancer UK","University of York","Leeds Biomedical Research Centre","Swedish Research Council","Wellcome Trust","UK Research and Innovation","University of Cambridge","Biotechnology and Biological Sciences Research Council"],"pagination":["116770"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12847853"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["45(1)"],"pubmed_abstract":["Despite rapid advances in mapping genetic drivers and gene expression changes in hematopoietic stem cells (HSCs), few studies exist at the protein level. We perform a deep, multi-omics characterization (epigenome, transcriptome, and proteome) of HSCs in a mouse model carrying a loss-of-function mutation in Tet2, a driver of increased self-renewal in blood cancers. Using state-of-the-art, multiplexed, low-input mass spectrometry (MS)-based proteomics, we profile TET2-deficient (Tet2<sup>-/-</sup>) HSCs, revealing previously unrecognized molecular processes that define the pre-leukemic HSC molecular landscape. Specifically, we obtain more accurate stratification of wild-type and Tet2<sup>-/-</sup> HSCs than transcriptomic approaches and identify extracellular matrix (ECM) molecules as being "],"journal":["Cell reports"],"pubmed_title":["Low-input proteomics identifies vWF as a negative regulator of Tet2 mutant hematopoietic stem cell expansion."],"pmcid":["PMC12847853"],"funding_grant_id":["2021-00185","MR/Y011945/1","C1163/A21762","NIHR203331","MC_PC_17230","ERC-2016-STG-715371","BB/T007222/1","MR/V005502/1","203151/Z/16/Z","MC_PC_21043","715371","206328/Z/17/Z","100008"],"pubmed_authors":["Rubio Lara JA","Vasey G","Belmonte M","Shepherd MS","Ahmed S","Hall BA","Kooi H","Milek J","MacDonald S","Wilson NK","Vijayabaskar MS","Theeuwes B","Brackenbury WJ","Cosme LC","Roumeliotis TI","Jassinskaja M","Chi Che JL","Bennett E","Holding AN","Gottgens B","Hogg AJ","Gonka M","Elberfeld S","Kent DG","Kucinski I","Bode D","Cull AH","Choudhary J","Yamazaki S"],"additional_accession":[]},"is_claimable":false,"name":"Low-input proteomics identifies vWF as a negative regulator of Tet2 mutant hematopoietic stem cell expansion.","description":"Despite rapid advances in mapping genetic drivers and gene expression changes in hematopoietic stem cells (HSCs), few studies exist at the protein level. We perform a deep, multi-omics characterization (epigenome, transcriptome, and proteome) of HSCs in a mouse model carrying a loss-of-function mutation in Tet2, a driver of increased self-renewal in blood cancers. Using state-of-the-art, multiplexed, low-input mass spectrometry (MS)-based proteomics, we profile TET2-deficient (Tet2<sup>-/-</sup>) HSCs, revealing previously unrecognized molecular processes that define the pre-leukemic HSC molecular landscape. Specifically, we obtain more accurate stratification of wild-type and Tet2<sup>-/-</sup> HSCs than transcriptomic approaches and identify extracellular matrix (ECM) molecules as being ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-09T07:26:28.687Z","creation":"2026-06-09T03:12:04.987Z"},"accession":"S-EPMC12847853","cross_references":{"pubmed":["41447537"],"doi":["10.1016/j.celrep.2025.116770"]}}