biostudies-literatureUnknown17(1)Conde EIL-33 is an inflammatory cytokine contributing to asthma, Chronic Obstructive Pulmonary Disease (COPD), and autoimmune diseases. Although recent studies suggest that IL-33 can induce the generation of autoantibodies, the role of IL-33 on B cell maturation and tolerance is poorly understood. Here, by inducing systemic overexpression of IL-33 in mice, we show that this cytokine induces the IL-5 and CD4 T cell-dependent accumulation of plasmablasts and plasma cells of all isotypes in the spleen, and leads to an increased antibody production. IL-33 also disrupts splenic architecture and elevates autoantibody production, indicating a break in peripheral tolerance. Consistently, elevated levels of IL-33 exacerbate autoantibody production, kidney damage, and decrease survival in a mouse model of Nature communications1102https://www.ebi.ac.uk/biostudies/studies/S-EPMC12852098biostudies-literatureIL-33 expands plasma cells, disrupts germinal centers, and increases autoantibody production in mice.PMC12852098Conde EAsrat SOrengo JMGayvert KYarilin DMurphy AJVecchione ALimnander ALim WKBen LHHuang SBirchard DPedraza PSleeman MATasker CfalseIL-33 expands plasma cells, disrupts germinal centers, and increases autoantibody production in mice.IL-33 is an inflammatory cytokine contributing to asthma, Chronic Obstructive Pulmonary Disease (COPD), and autoimmune diseases. Although recent studies suggest that IL-33 can induce the generation of autoantibodies, the role of IL-33 on B cell maturation and tolerance is poorly understood. Here, by inducing systemic overexpression of IL-33 in mice, we show that this cytokine induces the IL-5 and CD4 T cell-dependent accumulation of plasmablasts and plasma cells of all isotypes in the spleen, and leads to an increased antibody production. IL-33 also disrupts splenic architecture and elevates autoantibody production, indicating a break in peripheral tolerance. Consistently, elevated levels of IL-33 exacerbate autoantibody production, kidney damage, and decrease survival in a mouse model of 2025-01-01T00:00:00Z2025 Dec2026-06-16T07:11:45.106Z2026-06-16T03:09:46.712ZS-EPMC128520984143648810.1038/s41467-025-67867-2