biostudies-literatureUnknown18Bispo MEnzymes within the cholesterol biosynthesis pathway, particularly those in post-squalene biosynthesis, have been linked to abnormal neurodevelopment. Alterations of individual enzymes manifest unique brain phenotypes, suggesting each enzyme has distinct roles within the mammalian neural cell. However, a comprehensive characterization of cholesterol biosynthesis enzymes to understand these differences has yet to be fully obtained. Therefore, this study aimed to contribute to this growing body of knowledge by characterizing the subcellular localization of the cholesterol biosynthesis enzyme Hydroxysteroid-17-beta7 (Hsd17b7) within a mammalian neural cell line. Using mouse Neuro2a cells, we compared expression patterns between both endogenous Hsd17b7 and GFP-tagged constructs. Using confocal Frontiers in molecular neuroscience1639803https://www.ebi.ac.uk/biostudies/studies/S-EPMC12852399biostudies-literatureHsd17b7 undergoes dynamic subcellular localization during Neuro2a differentiation.PMC12852399Driver AMBispo MBrague JCPennay MfalseHsd17b7 undergoes dynamic subcellular localization during Neuro2a differentiation.Enzymes within the cholesterol biosynthesis pathway, particularly those in post-squalene biosynthesis, have been linked to abnormal neurodevelopment. Alterations of individual enzymes manifest unique brain phenotypes, suggesting each enzyme has distinct roles within the mammalian neural cell. However, a comprehensive characterization of cholesterol biosynthesis enzymes to understand these differences has yet to be fully obtained. Therefore, this study aimed to contribute to this growing body of knowledge by characterizing the subcellular localization of the cholesterol biosynthesis enzyme Hydroxysteroid-17-beta7 (Hsd17b7) within a mammalian neural cell line. Using mouse Neuro2a cells, we compared expression patterns between both endogenous Hsd17b7 and GFP-tagged constructs. Using confocal 2025-01-01T00:00:00Z20252026-06-19T04:47:54.479Z2026-06-19T03:07:31.902ZS-EPMC128523994162525610.3389/fnmol.2025.1639803