{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(1)"],"submitter":["Nguyen VT"],"pubmed_abstract":["Photoacoustic microscopy (PAM) systems often face challenges in simultaneously achieving high speed, high resolution, high sensitivity, and a large field of view (FOV). To address this challenge, we have developed dual-channel PAM (DC-PAM) that can expand the FOV without compromising the imaging speed, detection sensitivity, or spatial resolution. DC-PAM has two identical, independent channels of laser excitation and acoustic detection. It exploits two facets of a single hexagon scanner to concurrently steer the dual excitation laser beams and the resultant acoustic waves. DC-PAM achieves an ultra-wide FOV of 22.5 × 24 mm² with a total functional imaging time of ~15 s. Proof-of-concept experiments were conducted using DC-PAM on freely-swimming zebrafish, hypoxia-challenged mice, and sleepi"],"journal":["Light, science & applications"],"pagination":["91"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12852836"],"repository":["biostudies-literature"],"pubmed_title":["Dual-channel high-speed functional photoacoustic microscopy with ultra-wide field of view."],"pmcid":["PMC12852836"],"pubmed_authors":["Nguyen VT","Yao J","Li J","Jayasundara N","Delia J","Vu T","Cho SW","DiSpirito A","Taboada C","Menozzi L"],"additional_accession":[]},"is_claimable":false,"name":"Dual-channel high-speed functional photoacoustic microscopy with ultra-wide field of view.","description":"Photoacoustic microscopy (PAM) systems often face challenges in simultaneously achieving high speed, high resolution, high sensitivity, and a large field of view (FOV). To address this challenge, we have developed dual-channel PAM (DC-PAM) that can expand the FOV without compromising the imaging speed, detection sensitivity, or spatial resolution. DC-PAM has two identical, independent channels of laser excitation and acoustic detection. It exploits two facets of a single hexagon scanner to concurrently steer the dual excitation laser beams and the resultant acoustic waves. DC-PAM achieves an ultra-wide FOV of 22.5 × 24 mm² with a total functional imaging time of ~15 s. Proof-of-concept experiments were conducted using DC-PAM on freely-swimming zebrafish, hypoxia-challenged mice, and sleepi","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-17T07:00:41.136Z","creation":"2026-06-17T03:10:34.812Z"},"accession":"S-EPMC12852836","cross_references":{"pubmed":["41605894"],"doi":["10.1038/s41377-025-02114-3"]}}