{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(1)"],"submitter":["Dong X"],"pubmed_abstract":["Serial multi-omic analysis of proteome, phosphoproteome, and glycoproteome is pivotal for elucidating drug mechanisms, discovering biomarkers, and identifying therapeutic targets. However, simultaneous multi-level post-translational modifications (PTMs) analysis via parallel processing is hampered by laborious, time-consuming procedures and inconsistent reproducibility. We present an integrated Multi-level PTMs-Proteomic Enrichment platform (MuPPE), enabling sequential glycoproteome, phosphoproteome, and proteome analysis from single biological samples. It combines protein aggregation capture with on-bead digestion and tandem enrichment, achieving superior reproducibility (CV 12.3% vs 17.6% conventional methods) while reducing processing time by 87.5% (4 hours vs 32 hours). MuPPE also enha"],"journal":["Nature communications"],"pagination":["948"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12852849"],"repository":["biostudies-literature"],"pubmed_title":["A versatile platform for sequential glyco-, phospho-, and proteomics with multi-PTMs integration."],"pmcid":["PMC12852849"],"pubmed_authors":["Li X","Ding X","Yang Y","Dong X","Liang X","Cui Y","Du G","Chen C","Shen Y","Xiong F"],"additional_accession":[]},"is_claimable":false,"name":"A versatile platform for sequential glyco-, phospho-, and proteomics with multi-PTMs integration.","description":"Serial multi-omic analysis of proteome, phosphoproteome, and glycoproteome is pivotal for elucidating drug mechanisms, discovering biomarkers, and identifying therapeutic targets. However, simultaneous multi-level post-translational modifications (PTMs) analysis via parallel processing is hampered by laborious, time-consuming procedures and inconsistent reproducibility. We present an integrated Multi-level PTMs-Proteomic Enrichment platform (MuPPE), enabling sequential glycoproteome, phosphoproteome, and proteome analysis from single biological samples. It combines protein aggregation capture with on-bead digestion and tandem enrichment, achieving superior reproducibility (CV 12.3% vs 17.6% conventional methods) while reducing processing time by 87.5% (4 hours vs 32 hours). MuPPE also enha","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-10T09:05:36.73Z","creation":"2026-06-10T03:11:36.297Z"},"accession":"S-EPMC12852849","cross_references":{"pubmed":["41605919"],"doi":["10.1038/s41467-025-68270-7"]}}