biostudies-literatureUnknown13(1)Polat M<h4>Significance</h4>Although the transmission of auditory information in the brain has been extensively studied, the mechanism underlying fine auditory discrimination remains incompletely understood. The basal region of the ventromedial nucleus of the thalamus (bVM) has recently been found to convey frequency-specific auditory information to the primary auditory cortex (A1). Inhibition of the bVM significantly impairs fine auditory discrimination in mice. These findings indicate that the bVM plays an important role in frequency information processing. However, direct functional evidence for tonotopic organization within the bVM is still lacking.<h4>Aim</h4>We aimed to investigate whether bVM neurons exhibit a spatially ordered frequency preference, using a simple yet efficient <i>in vivo<Neurophotonics015008https://www.ebi.ac.uk/biostudies/studies/S-EPMC12853032biostudies-literatureTonotopic organization in the basal region of the ventromedial nucleus of the thalamus revealed by fiber photometry recording.PMC12853032Polat MTao JChen YChen XLi SCZhao ZfalseTonotopic organization in the basal region of the ventromedial nucleus of the thalamus revealed by fiber photometry recording.<h4>Significance</h4>Although the transmission of auditory information in the brain has been extensively studied, the mechanism underlying fine auditory discrimination remains incompletely understood. The basal region of the ventromedial nucleus of the thalamus (bVM) has recently been found to convey frequency-specific auditory information to the primary auditory cortex (A1). Inhibition of the bVM significantly impairs fine auditory discrimination in mice. These findings indicate that the bVM plays an important role in frequency information processing. However, direct functional evidence for tonotopic organization within the bVM is still lacking.<h4>Aim</h4>We aimed to investigate whether bVM neurons exhibit a spatially ordered frequency preference, using a simple yet efficient <i>in vivo<2026-01-01T00:00:00Z2026 Jan2026-06-18T05:46:59.871Z2026-06-18T03:07:55.419ZS-EPMC128530324162548210.1117/1.NPh.13.1.015008