{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Wang L"],"pubmed_abstract":["<h4>Background</h4>Insufficient infiltration of CD8<sup>+</sup> T cells in the tumor microenvironment (TME) critically restricts antitumor immunity and cancer immunotherapy efficacy. The purpose of this study was to identify novel tumor cell-intrinsic regulators of T-cell infiltration and to elucidate their mechanisms of action.<h4>Methods</h4>We performed a genome-wide Sleeping Beauty transposon mutagenesis screen in murine breast cancer models. Protein-protein interactions were identified by mass spectrometry and validated by co-immunoprecipitation. Gene and protein expression levels were assessed by reverse transcription and quantitative PCR and western blotting. T-cell infiltration and function were evaluated using flow cytometry, immunohistochemistry (IHC), multiplex IHC, and by analy"],"journal":["Journal for immunotherapy of cancer"],"pagination":["e013142"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12853559"],"repository":["biostudies-literature"],"pubmed_title":["DUSP22 dephosphorylates LGALS1 to enhance T cell-driven antitumor immunity."],"pmcid":["PMC12853559"],"pubmed_authors":["Shao F","Li J","Zhou N","Ma L","Guo Y","Deng CX","Lei H","Feng Y","Sun W","Dai Y","Wang L","Miao K","Li Y","Pan J","Luo P","Hong Y","Huang X","Zeng J","Chen W","Chen S","Sun H","Xu X","Zhang A"],"additional_accession":[]},"is_claimable":false,"name":"DUSP22 dephosphorylates LGALS1 to enhance T cell-driven antitumor immunity.","description":"<h4>Background</h4>Insufficient infiltration of CD8<sup>+</sup> T cells in the tumor microenvironment (TME) critically restricts antitumor immunity and cancer immunotherapy efficacy. The purpose of this study was to identify novel tumor cell-intrinsic regulators of T-cell infiltration and to elucidate their mechanisms of action.<h4>Methods</h4>We performed a genome-wide Sleeping Beauty transposon mutagenesis screen in murine breast cancer models. Protein-protein interactions were identified by mass spectrometry and validated by co-immunoprecipitation. Gene and protein expression levels were assessed by reverse transcription and quantitative PCR and western blotting. T-cell infiltration and function were evaluated using flow cytometry, immunohistochemistry (IHC), multiplex IHC, and by analy","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-09T05:45:55.699Z","creation":"2026-06-09T03:08:03.057Z"},"accession":"S-EPMC12853559","cross_references":{"pubmed":["41611244"],"doi":["10.1136/jitc-2025-013142"]}}