biostudies-literatureUnknown26(1)Tariq HPlant adaptation to environmental stress involves tightly regulated cellular, molecular, and biochemical responses. Among these, microbe-assisted strategies have gained attention, particularly the role of the plant microbiome (phytomicrobiome) in promoting growth and stress resilience. Soybean (Glycine max), a major agricultural crop, actively recruits beneficial microbes through root-secreted secondary metabolites, fostering symbiotic interactions with endophytic bacteria. However, the direct and indirect impacts of root-associated endophytes on plant development remain incompletely understood. In this study, we investigated three Bacillus strains (HT1, HT2, and HT3) isolated previously from the soybean root microbiome.16 S rRNA analysis indicates that HT1 and HT2 are closely related to tBMC plant biology178https://www.ebi.ac.uk/biostudies/studies/S-EPMC12853601biostudies-literaturePlant growth-promoting Bacillus strains modulate early soybean development via proteome remodeling.PMC12853601Geddes-McAlister JDutilleul PGeitmann ATariq HSmith DLfalsePlant growth-promoting Bacillus strains modulate early soybean development via proteome remodeling.Plant adaptation to environmental stress involves tightly regulated cellular, molecular, and biochemical responses. Among these, microbe-assisted strategies have gained attention, particularly the role of the plant microbiome (phytomicrobiome) in promoting growth and stress resilience. Soybean (Glycine max), a major agricultural crop, actively recruits beneficial microbes through root-secreted secondary metabolites, fostering symbiotic interactions with endophytic bacteria. However, the direct and indirect impacts of root-associated endophytes on plant development remain incompletely understood. In this study, we investigated three Bacillus strains (HT1, HT2, and HT3) isolated previously from the soybean root microbiome.16 S rRNA analysis indicates that HT1 and HT2 are closely related to t2025-01-01T00:00:00Z2025 Dec2026-06-08T03:31:38.295Z2026-06-08T03:14:41.493ZS-EPMC128536014145588710.1186/s12870-025-07972-y