biostudies-literatureAllegretti MMinistero della SaluteFondazione AIRC per la ricerca sul cancro ETS26https://www.ebi.ac.uk/biostudies/studies/S-EPMC12853808biostudies-literatureUnknown45(1)<h4>Background</h4>Cancer resistance is one of the major challenges in oncology, often resulting in disease relapse and poor patient outcomes. Within the RNA family, microRNAs (miRNAs) regulate core biological processes and have been recognized also as critical contributors of tumor resistance and therapy failure. Being pivotal, they are increasingly exploited as biomarkers in various settings. Although in silico analyses facilitate miRNAs identification, PCR-based approaches remain essential to validate their expression. Currently, a plethora of well-established, single-target methods exist but multiplex detection from the same input have been only rarely explored.<h4>Methods</h4>We present miRquad, the first-in-class digital PCR (dPCR) TaqMan™ multiplex clinical research assay for miRNA Journal of experimental & clinical cancer research : CRmiRquad: first-in-class dPCR multiplex TaqMan™ Advanced clinical research assay for microRNA detection in head and neck cancer.PMC12853808IG 29040PNC E3-2022-23683266Stevens JFPellini RMoccia TUrbani GMaestro RDe Summa SPisani AAzzariti AGanci FArbustini EUrtis MDi Gennaro ESigalotti LGagliardi SAllegretti MDe Pascale VCiliberto GFiori SBlandino GMuti PCMJoun DJCiardiello CBudillon AFerrero SBeltramini GAfalsemiRquad: first-in-class dPCR multiplex TaqMan™ Advanced clinical research assay for microRNA detection in head and neck cancer.<h4>Background</h4>Cancer resistance is one of the major challenges in oncology, often resulting in disease relapse and poor patient outcomes. Within the RNA family, microRNAs (miRNAs) regulate core biological processes and have been recognized also as critical contributors of tumor resistance and therapy failure. Being pivotal, they are increasingly exploited as biomarkers in various settings. Although in silico analyses facilitate miRNAs identification, PCR-based approaches remain essential to validate their expression. Currently, a plethora of well-established, single-target methods exist but multiplex detection from the same input have been only rarely explored.<h4>Methods</h4>We present miRquad, the first-in-class digital PCR (dPCR) TaqMan™ multiplex clinical research assay for miRNA 2025-01-01T00:00:00Z2025 Dec2026-06-17T03:22:49.283Z2026-06-17T03:10:43.878ZS-EPMC128538084142203010.1186/s13046-025-03590-6