{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["22(1)"],"submitter":["Lyons FMT"],"pubmed_abstract":["Malaria parasite fertilisation occurs within the Anopheles mosquito midgut. Interventions that inhibit parasite fertilisation prevent ongoing transmission and are important for malaria elimination efforts. Pfs48/45 and Pfs230 are two leading transmission-blocking vaccine candidates. Both proteins form a complex on the surface of sexual stage parasites and are essential for male fertility. Here we have identified nanobodies against Pfs48/45 that recognise gametocytes and have strong transmission-reducing activity. The crystal structure of our most potent nanobody in complex with Pfs48/45 reveals it binds a distinct epitope to TB31F, a leading transmission-blocking monoclonal antibody but to similar epitopes as RUPA-44 and RUPA-117. These results demonstrate the potential of nanobodies as a "],"journal":["PLoS pathogens"],"pagination":["e1013884"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12858062"],"repository":["biostudies-literature"],"pubmed_title":["Pfs48/45 nanobodies block Plasmodium falciparum transmission."],"pmcid":["PMC12858062"],"pubmed_authors":["Chmielewski J","Zeglinski K","Dietrich MH","Gouil Q","Lyons FMT","Tong J","Adair A","Gabriela M","Tham WH","Chan LJ"],"additional_accession":[]},"is_claimable":false,"name":"Pfs48/45 nanobodies block Plasmodium falciparum transmission.","description":"Malaria parasite fertilisation occurs within the Anopheles mosquito midgut. Interventions that inhibit parasite fertilisation prevent ongoing transmission and are important for malaria elimination efforts. Pfs48/45 and Pfs230 are two leading transmission-blocking vaccine candidates. Both proteins form a complex on the surface of sexual stage parasites and are essential for male fertility. Here we have identified nanobodies against Pfs48/45 that recognise gametocytes and have strong transmission-reducing activity. The crystal structure of our most potent nanobody in complex with Pfs48/45 reveals it binds a distinct epitope to TB31F, a leading transmission-blocking monoclonal antibody but to similar epitopes as RUPA-44 and RUPA-117. These results demonstrate the potential of nanobodies as a ","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-06-14T05:44:41.175Z","creation":"2026-06-14T03:09:17.427Z"},"accession":"S-EPMC12858062","cross_references":{"pubmed":["41592139"],"doi":["10.1371/journal.ppat.1013884"]}}