{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["25(2)"],"submitter":["Sanchez CD"],"funding":["NIH NIAID","NIH NCI"],"pubmed_abstract":["Although the plasma membrane (PM) is among the most biologically important and therapeutically targeted cellular compartments, it is among the most challenging to faithfully capture using proteomic approaches. The quality of quantitative surfaceomics data depends heavily on the effectiveness of the cell surface enrichment used during sample preparation. Enrichment improves sensitivity for low abundance PM proteins and ensures that the changes detected reflect PM expression changes rather than whole cell changes. Cell surface biotinylation with PM-impermeable, amine-reactive reagents is a facile, accessible, and unbiased approach to enrich PM proteins. However, it results in unexpectedly high contamination with intracellular proteins, reducing its utility. We report that biotinylating human"],"journal":["Molecular & cellular proteomics : MCP"],"pagination":["101503"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12859484"],"repository":["biostudies-literature"],"pubmed_title":["Improved T Cell Surfaceomics by Depleting Intracellularly Labelled Dead Cells."],"pmcid":["PMC12859484"],"pubmed_authors":["Sanchez CD","Krisko B","Minasyan M","Valenzuela O","Mkhikian H","Ahmmed B","Balakrishnan A","Witchey L","Pak A"],"additional_accession":[]},"is_claimable":false,"name":"Improved T Cell Surfaceomics by Depleting Intracellularly Labelled Dead Cells.","description":"Although the plasma membrane (PM) is among the most biologically important and therapeutically targeted cellular compartments, it is among the most challenging to faithfully capture using proteomic approaches. The quality of quantitative surfaceomics data depends heavily on the effectiveness of the cell surface enrichment used during sample preparation. Enrichment improves sensitivity for low abundance PM proteins and ensures that the changes detected reflect PM expression changes rather than whole cell changes. Cell surface biotinylation with PM-impermeable, amine-reactive reagents is a facile, accessible, and unbiased approach to enrich PM proteins. However, it results in unexpectedly high contamination with intracellular proteins, reducing its utility. We report that biotinylating human","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-11T06:01:22.846Z","creation":"2026-06-11T03:12:01.884Z"},"accession":"S-EPMC12859484","cross_references":{"pubmed":["41453615"],"doi":["10.1016/j.mcpro.2025.101503"]}}