{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chang Y"],"funding":["Yunnan Major Scientific and Technological Projects"],"pagination":["72"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12859952"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(1)"],"pubmed_abstract":["<h4>Background</h4>Rapidly detecting drug-resistant Mycobacterium tuberculosis (MTB) is essential for effective tuberculosis (TB) treatment. We have developed a one-tube nested fluorescence melting curve assay (OTN-FMCA) that allows rapid and simultaneous detection of resistance to rifampicin, isoniazid, fluoroquinolones, and bedaquiline.<h4>Results</h4>The OTN-FMCA uses nested PCR and locked nucleic acid (LNA)-modified probes to identify 44 mutations in six key genes (rpoB, katG, inhA, gyrA, gyrB, and atpE), achieving a detection limit of 5 copies per microliter and identifying up to 10% heterogeneous resistance. When tested on 198 culture-positive isolates, OTN-FMCA demonstrated high concordance with microarray susceptibility testing, with 100% sensitivity and 96.89% specificity. Further"],"journal":["BMC microbiology"],"pubmed_title":["A novel one-tube nested fluorescence melting curve analysis for rapid detection of drug-resistant Mycobacterium tuberculosis."],"pmcid":["PMC12859952"],"funding_grant_id":["(202202AG050013, 202302AA310044, 202405AJ310002, and 202502AA310025"],"pubmed_authors":["Xia X","Liu L","Zhang H","Feng Y","Zhang M","Chang Y","Yang C","Li X","Zhou C","Diao T","Tu Y","Lei P","Liu G","Wu X"],"additional_accession":[]},"is_claimable":false,"name":"A novel one-tube nested fluorescence melting curve analysis for rapid detection of drug-resistant Mycobacterium tuberculosis.","description":"<h4>Background</h4>Rapidly detecting drug-resistant Mycobacterium tuberculosis (MTB) is essential for effective tuberculosis (TB) treatment. We have developed a one-tube nested fluorescence melting curve assay (OTN-FMCA) that allows rapid and simultaneous detection of resistance to rifampicin, isoniazid, fluoroquinolones, and bedaquiline.<h4>Results</h4>The OTN-FMCA uses nested PCR and locked nucleic acid (LNA)-modified probes to identify 44 mutations in six key genes (rpoB, katG, inhA, gyrA, gyrB, and atpE), achieving a detection limit of 5 copies per microliter and identifying up to 10% heterogeneous resistance. When tested on 198 culture-positive isolates, OTN-FMCA demonstrated high concordance with microarray susceptibility testing, with 100% sensitivity and 96.89% specificity. Further","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-16T07:17:58.603Z","creation":"2026-06-16T03:10:08.919Z"},"accession":"S-EPMC12859952","cross_references":{"pubmed":["41420205"],"doi":["10.1186/s12866-025-04601-4"]}}