biostudies-literatureUnknown60Xing MTemporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease that lacks effective treatment options. The inflammatory microenvironment caused by excessive reactive oxygen species (ROS) in TMJ-OA leads to chondrocyte apoptosis, extracellular matrix (ECM) degradation, and abnormal cartilage metabolism. Herein, we innovatively constructed a manganese-nitrogen-carbon single-atom nanozymes (Mn-NC SAzymes) - chitosan composite hydrogel system. A coordination structure of Mn-N₄ was proposed based on X-ray absorption fine structure spectra. The composite hydrogel achieves an integration of atomic level catalytic design and a localized delivery system. It fully leverages the synergistic dual enzyme-like activities of the Mn - NC SAzyme and the active repair properties of chiBioactive materials216-242https://www.ebi.ac.uk/biostudies/studies/S-EPMC12860705biostudies-literatureNanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway.PMC12860705Liu XZhu MQian WQiu JXing MFeng JCao YChen SYe KfalseNanozyme hydrogels remodel pathological microenvironment for temporomandibular joint osteoarthritis therapy via inhibiting MAPK signal pathway.Temporomandibular joint osteoarthritis (TMJ-OA) is a degenerative disease that lacks effective treatment options. The inflammatory microenvironment caused by excessive reactive oxygen species (ROS) in TMJ-OA leads to chondrocyte apoptosis, extracellular matrix (ECM) degradation, and abnormal cartilage metabolism. Herein, we innovatively constructed a manganese-nitrogen-carbon single-atom nanozymes (Mn-NC SAzymes) - chitosan composite hydrogel system. A coordination structure of Mn-N₄ was proposed based on X-ray absorption fine structure spectra. The composite hydrogel achieves an integration of atomic level catalytic design and a localized delivery system. It fully leverages the synergistic dual enzyme-like activities of the Mn - NC SAzyme and the active repair properties of chi2026-01-01T00:00:00Z2026 Jun2026-06-18T05:52:39.402Z2026-06-18T03:07:27.03ZS-EPMC128607054163082610.1016/j.bioactmat.2026.01.031