{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Zheng S"],"pubmed_abstract":["The lack of a cell-based screening method limits urate-lowering drug development. A novel method combining aptamer sensor array (ASA), exonuclease III (Exo III)- powered 3D DNA walker (DW), and linear discriminant analysis (LDA) was developed for detecting uric acid (UA) in cell lysates, referred to as ASA-Exo III-DW-LDA. Three aptamers (Apts) with different affinities for UA and its structurally similar compound, xanthine (Xan), were used to design the ASA. The combination of ASA and Exo III-DW enabled the detection of UA at the picomolar level, whereas LDA was employed to differentiate UA signals from the mixed signals of UA and Xan. Significantly, Pearson correlation analysis revealed a strong correlation between our method and the <sup>14</sup>C radioactive labeling method for urate an"],"journal":["Journal of pharmaceutical analysis"],"pagination":["101345"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12860932"],"repository":["biostudies-literature"],"pubmed_title":["A novel method for screening antihyperuricemic drugs by combining aptamer sensor array, exonuclease III-DNA walker and linear discriminant analysis."],"pmcid":["PMC12860932"],"pubmed_authors":["Shao H","Liu L","Ke J","Zheng S","Pang J","Chen H","Zheng F","Zhang R","Zhao Z"],"additional_accession":[]},"is_claimable":false,"name":"A novel method for screening antihyperuricemic drugs by combining aptamer sensor array, exonuclease III-DNA walker and linear discriminant analysis.","description":"The lack of a cell-based screening method limits urate-lowering drug development. A novel method combining aptamer sensor array (ASA), exonuclease III (Exo III)- powered 3D DNA walker (DW), and linear discriminant analysis (LDA) was developed for detecting uric acid (UA) in cell lysates, referred to as ASA-Exo III-DW-LDA. Three aptamers (Apts) with different affinities for UA and its structurally similar compound, xanthine (Xan), were used to design the ASA. The combination of ASA and Exo III-DW enabled the detection of UA at the picomolar level, whereas LDA was employed to differentiate UA signals from the mixed signals of UA and Xan. Significantly, Pearson correlation analysis revealed a strong correlation between our method and the <sup>14</sup>C radioactive labeling method for urate an","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-14T21:02:02.414Z","creation":"2026-06-25T03:07:34.963Z"},"accession":"S-EPMC12860932","cross_references":{"pubmed":["41631245"],"doi":["10.1016/j.jpha.2025.101345"]}}