{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lee MY"],"funding":["NIDCR NIH HHS"],"pagination":["e014067"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12863322"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["<h4>Background</h4>Neoadjuvant PD-1/PD-L1 blockade yields robust efficacy in advanced cutaneous squamous cell carcinoma (cSCC), yet many patients fail to achieve a complete or major pathologic response. The reasons why some patients experience response but others do not are unclear.<h4>Methods</h4>We profiled cSCC specimens before, after 1 dose, and after 3-4 doses of PD-1/PD-L1 blockade to uncover resistance mechanisms and predict therapeutic response. In total, 27 patients across three cohorts, including two phase II trials, were studied. We created 1.7 mm tissue-core microarrays and performed single-cell spatial transcriptomics, including spatial clustering, gene-set enrichment, and spatial correlation analyses.<h4>Results</h4>After profiling all samples, six distinct spatial niches eme"],"journal":["Journal for immunotherapy of cancer"],"pubmed_title":["Single-cell spatial transcriptomics uncovers niches that govern response to PD-1/PD-L1 blockade in cutaneous squamous cell carcinoma."],"pmcid":["PMC12863322"],"funding_grant_id":["R35 DE030054"],"pubmed_authors":["Colevas AD","Shin JH","Martinez Ramirez L","Divi V","Zdravkovic S","Sherman AR","Sunwoo JB","Rahman M","Lee MY","Stepanek I"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell spatial transcriptomics uncovers niches that govern response to PD-1/PD-L1 blockade in cutaneous squamous cell carcinoma.","description":"<h4>Background</h4>Neoadjuvant PD-1/PD-L1 blockade yields robust efficacy in advanced cutaneous squamous cell carcinoma (cSCC), yet many patients fail to achieve a complete or major pathologic response. The reasons why some patients experience response but others do not are unclear.<h4>Methods</h4>We profiled cSCC specimens before, after 1 dose, and after 3-4 doses of PD-1/PD-L1 blockade to uncover resistance mechanisms and predict therapeutic response. In total, 27 patients across three cohorts, including two phase II trials, were studied. We created 1.7 mm tissue-core microarrays and performed single-cell spatial transcriptomics, including spatial clustering, gene-set enrichment, and spatial correlation analyses.<h4>Results</h4>After profiling all samples, six distinct spatial niches eme","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-15T00:52:33.199Z","creation":"2026-06-27T03:05:39.415Z"},"accession":"S-EPMC12863322","cross_references":{"pubmed":["41617396"],"doi":["10.1136/jitc-2025-014067"]}}