biostudies-literatureUnknown21(2)Rahman ZThis study investigates the insecticidal efficacy of Areca catechu nut extract against Aedes aegypti and Aedes albopictus, in response to increasing insecticide resistance, declining effectiveness of conventional agents, and environmental safety concerns. The primary objectives were to evaluate the adulticidal, ovicidal, and oviposition deterrent activities of methanolic A. catechu nut extract across a concentration range of 300-2000 ppm, and to identify its major bioactive constituents. Laboratory bioassays were conducted following World Health Organization protocols. The extract induced dose-dependent adult mortality, with LC50 values of 767.501 ppm for Ae. aegypti and 758.278 ppm for Ae. albopictus. Ovicidal assays showed progressive increases in egg mortality, reaching 100% at 1600 ppmPloS onee0341897https://www.ebi.ac.uk/biostudies/studies/S-EPMC12863473biostudies-literatureInsecticidal potential of Areca catechu nut extract against multiple life stages of Aedes aegypti and Aedes albopictus.PMC12863473Junaid OQLing LYIshak IHRahman ZBharathithasan MRavi RfalseInsecticidal potential of Areca catechu nut extract against multiple life stages of Aedes aegypti and Aedes albopictus.This study investigates the insecticidal efficacy of Areca catechu nut extract against Aedes aegypti and Aedes albopictus, in response to increasing insecticide resistance, declining effectiveness of conventional agents, and environmental safety concerns. The primary objectives were to evaluate the adulticidal, ovicidal, and oviposition deterrent activities of methanolic A. catechu nut extract across a concentration range of 300-2000 ppm, and to identify its major bioactive constituents. Laboratory bioassays were conducted following World Health Organization protocols. The extract induced dose-dependent adult mortality, with LC50 values of 767.501 ppm for Ae. aegypti and 758.278 ppm for Ae. albopictus. Ovicidal assays showed progressive increases in egg mortality, reaching 100% at 1600 ppm2026-01-01T00:00:00Z20262026-07-14T17:19:34.485Z2026-06-21T03:13:10.764ZS-EPMC128634734162817210.1371/journal.pone.0341897