{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(1)"],"submitter":["Maerz MD"],"funding":["National Institute of Allergy and Infectious Diseases","National Institutes of Health"],"pubmed_abstract":["γδ T cells expressing a Vδ1/3+ T cell receptor are enriched at mucosal surfaces, but their role in protection against Mycobacterium tuberculosis (Mtb) is largely unknown. We used multimodal single-cell RNA sequencing, mass cytometry, and flow cytometry to profile γδ T cells from human infants and macaques after protective vaccination with Mycobacterium bovis bacillus Calmette Guerin (BCG). A subset of Vδ1/3 T cells in BCG-vaccinated human infants shows evidence of clonal expansion and differentiation into Mtb-reactive cytotoxic effector cells. In macaques, intravenous BCG induces pro-inflammatory and cytotoxic responses to Mtb among Vδ1/3 T cells that are enriched in the airway compared to the blood. Finally, the frequency of cytokine-expressing Vδ1/3 T cells in the airway is associated with protection against Mtb challenge. Thus, Vδ1/3 T cells are activated by BCG and accumulate in the lung, where they upregulate cytotoxic and pro-inflammatory functions that may contribute to protective immunity against Mtb."],"journal":["Cell reports. Medicine"],"pagination":["102536"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12866138"],"repository":["biostudies-literature"],"pubmed_title":["BCG vaccination induces antibacterial effector functions among Vδ1/3 T cells that are associated with protection against tuberculosis."],"pmcid":["PMC12866138"],"pubmed_authors":["Bucsan AN","Bishop E","Roederer M","Layton ED","Wang C","Sutton MS","Seder RA","Maerz MD","Tian Z","Shalek AK","Darrah PA","Scriba TJ","Makatsa MS","Seshadri C"],"additional_accession":[]},"is_claimable":false,"name":"BCG vaccination induces antibacterial effector functions among Vδ1/3 T cells that are associated with protection against tuberculosis.","description":"γδ T cells expressing a Vδ1/3+ T cell receptor are enriched at mucosal surfaces, but their role in protection against Mycobacterium tuberculosis (Mtb) is largely unknown. We used multimodal single-cell RNA sequencing, mass cytometry, and flow cytometry to profile γδ T cells from human infants and macaques after protective vaccination with Mycobacterium bovis bacillus Calmette Guerin (BCG). A subset of Vδ1/3 T cells in BCG-vaccinated human infants shows evidence of clonal expansion and differentiation into Mtb-reactive cytotoxic effector cells. In macaques, intravenous BCG induces pro-inflammatory and cytotoxic responses to Mtb among Vδ1/3 T cells that are enriched in the airway compared to the blood. Finally, the frequency of cytokine-expressing Vδ1/3 T cells in the airway is associated with protection against Mtb challenge. Thus, Vδ1/3 T cells are activated by BCG and accumulate in the lung, where they upregulate cytotoxic and pro-inflammatory functions that may contribute to protective immunity against Mtb.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Jan","modification":"2026-07-02T03:15:06.076Z","creation":"2026-07-02T03:08:27.859Z"},"accession":"S-EPMC12866138","cross_references":{"pubmed":["41529694"],"doi":["10.1016/j.xcrm.2025.102536"]}}