biostudies-literatureSong YOut-standing Young Talents Program of Capital Medial UniversityChina National Natural Science FoundationBeijing Hospital Management Center "peak" talent training plan teamCapital's Funds for Health Improvement and ResearchBeijing Natural Science Foundation59https://www.ebi.ac.uk/biostudies/studies/S-EPMC12866310biostudies-literatureUnknown21(1)<h4>Rationale</h4>Chronic migraine is characterized by persistent trigeminal sensitization and neuroinflammation. however, the molecular mechanisms underlying its maintenance and mediate the therapeutic effects of acupuncture remian incompletely understood.<h4>Method</h4>A chronic migraine-like state was induced in mice by repeated dural inflammatory soup (IS), followed by electroacupuncture (EA). Behavioral hypersensitivity was assessed, and molecular changes in the spinal trigeminal nucleus caudalis (Sp5C) were analyzed using transcriptomic, biochemical, and functional approaches.<h4>Result</h4>Repeated inflammatory stimulation markedly increased CXCL13 and CXCR5 expression and ERK phosphorylation in the Sp5C, accompanied by mechanical allodynia, thermal hyperalgesia, glial activation, aChinese medicineElectroacupuncture alleviates migraine through CXCL13/CXCR5-mediated communication.PMC12866310B2207820741797234409CFH2024-2-2235DFL20241001823053897232270Liu LCao BYang XLiu YZhao LZhao SGe XPeng PLuo YLi BZhang CWei JSong YfalseElectroacupuncture alleviates migraine through CXCL13/CXCR5-mediated communication.<h4>Rationale</h4>Chronic migraine is characterized by persistent trigeminal sensitization and neuroinflammation. however, the molecular mechanisms underlying its maintenance and mediate the therapeutic effects of acupuncture remian incompletely understood.<h4>Method</h4>A chronic migraine-like state was induced in mice by repeated dural inflammatory soup (IS), followed by electroacupuncture (EA). Behavioral hypersensitivity was assessed, and molecular changes in the spinal trigeminal nucleus caudalis (Sp5C) were analyzed using transcriptomic, biochemical, and functional approaches.<h4>Result</h4>Repeated inflammatory stimulation markedly increased CXCL13 and CXCR5 expression and ERK phosphorylation in the Sp5C, accompanied by mechanical allodynia, thermal hyperalgesia, glial activation, a2026-01-01T00:00:00Z2026 Feb2026-07-15T11:22:37.14Z2026-07-04T03:12:18.006ZS-EPMC128663104163004710.1186/s13020-026-01338-8