{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Angiolillo S"],"funding":["2019 STARS Grants program","2021 STARS Grants program"],"pagination":["20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12868028"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["76(1)"],"pubmed_abstract":["The generation of engineered neurons via Neurogenin-2 (NGN2) overexpression, starting from human induced pluripotent stem cells (hiPSCs), is a powerful tool for modeling neurological diseases. However, using stabilized hiPSCs as a starting point significantly increases the time required to obtain a valuable human neuronal model in vitro. Here, we demonstrated that as little as 3 days of transient expression of reprogramming factors in human fibroblasts can unlock the ability of these cells to transdifferentiate into neurons upon overexpression of NGN2. We used single-cell transcriptomic data to dissect the distinct cell identities that emerge during reprogramming. We identified three distinct reprogramming intermediate populations responsive to NGN2-mediated neuronal induction and found th"],"journal":["Journal of molecular neuroscience : MN"],"pubmed_title":["Early Reprogramming Intermediates Enable Direct Neuronal Conversion Via NGN2."],"pmcid":["PMC12868028"],"funding_grant_id":["EnCOr","iNeurons"],"pubmed_authors":["Gesualdo A","Elvassore N","Angiolillo S","Laterza C","Frison R","Gagliano O","Qin W"],"additional_accession":[]},"is_claimable":false,"name":"Early Reprogramming Intermediates Enable Direct Neuronal Conversion Via NGN2.","description":"The generation of engineered neurons via Neurogenin-2 (NGN2) overexpression, starting from human induced pluripotent stem cells (hiPSCs), is a powerful tool for modeling neurological diseases. However, using stabilized hiPSCs as a starting point significantly increases the time required to obtain a valuable human neuronal model in vitro. Here, we demonstrated that as little as 3 days of transient expression of reprogramming factors in human fibroblasts can unlock the ability of these cells to transdifferentiate into neurons upon overexpression of NGN2. We used single-cell transcriptomic data to dissect the distinct cell identities that emerge during reprogramming. We identified three distinct reprogramming intermediate populations responsive to NGN2-mediated neuronal induction and found th","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-05T03:10:48.28Z","creation":"2026-07-05T03:08:25.779Z"},"accession":"S-EPMC12868028","cross_references":{"pubmed":["41632409"],"doi":["10.1007/s12031-025-02460-2"]}}