{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["70(2)"],"submitter":["Wu MC"],"funding":["This study was sypported by Japan Society for the Promotion of Science (JSPS) KAKENHI, Grant Numbers: JP20H05682 (K.T.), JP24K22131 (K.T.), JPJSCCA20240006 (A.M.), 25H01316 (A.M.), JP23K14083 (T.K.), JP25K18813 (T.K.), and 23K14528 (M.S.); Japan Agency for Medical Research and Development (AMED), Grant Number: JP23bm1223017h0001 (K.T.); Kaketsuken Research Grant (K.T.); The Joint Usage/Research Center Program on Institute for Life and Medical Sciences (LiMe), Kyoto University (K.T.); and The LiMe Grant 2024 and 2025, Kyoto University (T.K.)."],"pubmed_abstract":["Orthobornaviruses express X and the phosphoprotein (P) from a bicistronic X/P mRNA, and these proteins regulate polymerase activity. In mammalian orthobornaviruses, the 5' untranslated region (5' UTR) of the X/P mRNA controls the translational balance between X and P and thereby promotes efficient replication. Avian bornaviruses (ABVs) belong to two clades, clade-2 and -3, that differ in the structure and length of the 5' UTR of the X/P mRNA. However, the functional consequences of these differences remain unclear. Using reverse genetics, we generated chimeric viruses by reciprocally exchanging the 5' UTR of the X/P mRNA among clade 2 parrot bornavirus 5 (PaBV-5) and aquatic bird bornavirus 1 (ABBV-1) and clade 3 PaBV-4. In PaBV-5, a long 5' UTR with a stem-loop and an upstream ORF was req"],"journal":["Microbiology and immunology"],"pagination":["80-90"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12868937"],"repository":["biostudies-literature"],"pubmed_title":["Distinct 5' UTR Requirements for Translation of the Bicistronic X/P mRNA Among Avian Orthobornaviruses."],"pmcid":["PMC12868937"],"pubmed_authors":["Tomonaga K","Wu MC","Leacy A","Komorizono R","Susta L","Makino A","Sakai M","Kanda T"],"additional_accession":[]},"is_claimable":false,"name":"Distinct 5' UTR Requirements for Translation of the Bicistronic X/P mRNA Among Avian Orthobornaviruses.","description":"Orthobornaviruses express X and the phosphoprotein (P) from a bicistronic X/P mRNA, and these proteins regulate polymerase activity. In mammalian orthobornaviruses, the 5' untranslated region (5' UTR) of the X/P mRNA controls the translational balance between X and P and thereby promotes efficient replication. Avian bornaviruses (ABVs) belong to two clades, clade-2 and -3, that differ in the structure and length of the 5' UTR of the X/P mRNA. However, the functional consequences of these differences remain unclear. Using reverse genetics, we generated chimeric viruses by reciprocally exchanging the 5' UTR of the X/P mRNA among clade 2 parrot bornavirus 5 (PaBV-5) and aquatic bird bornavirus 1 (ABBV-1) and clade 3 PaBV-4. In PaBV-5, a long 5' UTR with a stem-loop and an upstream ORF was req","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Feb","modification":"2026-07-15T12:03:24.742Z","creation":"2026-07-04T03:12:44.557Z"},"accession":"S-EPMC12868937","cross_references":{"pubmed":["41355325"],"doi":["10.1111/1348-0421.70031"]}}