biostudies-literatureUnknownZhang FNIDDK NIH HHSNIAID NIH HHS<h4>Background and aims</h4>Hepatitis B virus (HBV) and hepatitis C virus (HCV) share transmission routes and often coinfect the liver, leading to accelerated liver disease progression. In the era of direct-acting antivirals (DAAs) for HCV, the clinical impact of coinfection is further complicated by reports of HBV reactivation following HCV cure. While HCV-induced interferon (IFN) responses are known to suppress HBV, the underlying mechanisms remain incompletely understood. This study aimed to investigate how HCV modulates HBV infection in the setting of coinfection and to identify strategies to prevent HBV reactivation post-HCV clearance.<h4>Approach and results</h4>We utilized a multicellular liver culture model composed of human-induced pluripotent stem cell (hiPSC)-derived hepatocytesHepatology (Baltimore, Md.)https://www.ebi.ac.uk/biostudies/studies/S-EPMC12869898biostudies-literatureIL1β signaling mediates the interaction between hepatitis B and C viruses.PMC12869898R00 AI141742DP2 AI170515R01 DK125100Zhang FWu JKwong ACFeng ZHa EHZhao YWang LBian LNing LDao Thi VLZhang YSanchez EChen WFoo SSO'Shaughnessy KWang XChen SYu YWu XfalseIL1β signaling mediates the interaction between hepatitis B and C viruses.<h4>Background and aims</h4>Hepatitis B virus (HBV) and hepatitis C virus (HCV) share transmission routes and often coinfect the liver, leading to accelerated liver disease progression. In the era of direct-acting antivirals (DAAs) for HCV, the clinical impact of coinfection is further complicated by reports of HBV reactivation following HCV cure. While HCV-induced interferon (IFN) responses are known to suppress HBV, the underlying mechanisms remain incompletely understood. This study aimed to investigate how HCV modulates HBV infection in the setting of coinfection and to identify strategies to prevent HBV reactivation post-HCV clearance.<h4>Approach and results</h4>We utilized a multicellular liver culture model composed of human-induced pluripotent stem cell (hiPSC)-derived hepatocytes2025-01-01T00:00:00Z2025 Sep2026-07-15T06:12:25.89Z2026-06-30T03:21:37.394ZS-EPMC128698984093819810.1097/hep.000000000000152510.1097/HEP.0000000000001525