biostudies-literatureUnknown26(1)Cai H<h4>Background</h4>Liver hepatocellular carcinoma (LIHC) is one of the most common and aggressive malignancies worldwide, with high mortality rates. PLOD family genes (PLOD1, PLOD2, and PLOD3) play a pivotal role in extracellular matrix (ECM) remodeling, which contributes to cancer progression and metastasis. This study explores the diagnostic, prognostic, and therapeutic potential of PLOD1, PLOD2, and PLOD3 in LIHC.<h4>Materials and methods</h4>Seven LIHC cell lines and five normal liver tissue cell lines were cultured. RT-qPCR was used to assess the expression of PLOD genes. The GSCA and TCGA databases were used for gene expression, pathway analysis, survival, and enrichment analysis. Promoter methylation was analyzed via GSCA and OncoDB, and mutational and CNV analyses were conducted usBMC cancer172https://www.ebi.ac.uk/biostudies/studies/S-EPMC12870100biostudies-literatureThe role of PLOD family genes in liver hepatocellular carcinoma: from mechanisms to therapeutic potential.PMC12870100Chen JHu GFeng HCai HBi YfalseThe role of PLOD family genes in liver hepatocellular carcinoma: from mechanisms to therapeutic potential.<h4>Background</h4>Liver hepatocellular carcinoma (LIHC) is one of the most common and aggressive malignancies worldwide, with high mortality rates. PLOD family genes (PLOD1, PLOD2, and PLOD3) play a pivotal role in extracellular matrix (ECM) remodeling, which contributes to cancer progression and metastasis. This study explores the diagnostic, prognostic, and therapeutic potential of PLOD1, PLOD2, and PLOD3 in LIHC.<h4>Materials and methods</h4>Seven LIHC cell lines and five normal liver tissue cell lines were cultured. RT-qPCR was used to assess the expression of PLOD genes. The GSCA and TCGA databases were used for gene expression, pathway analysis, survival, and enrichment analysis. Promoter methylation was analyzed via GSCA and OncoDB, and mutational and CNV analyses were conducted us2026-01-01T00:00:00Z2026 Jan2026-07-15T07:40:01.306Z2026-07-01T03:07:51.178ZS-EPMC128701004149116610.1186/s12885-025-15472-3