biostudies-literatureVazquez-Ucha JCCarlos III Health InstituteCentro de Investigación Biotecnológica en Red de Enfermedades InfecciosasAxencia Galega de InnovaciónMinisterio de Ciencia e InnovaciónMinistério da Ciência, Tecnologia e Ensino SuperiorFundacao para a Ciencia e a TecnologiaEuropean Commission101711https://www.ebi.ac.uk/biostudies/studies/S-EPMC12870466biostudies-literatureUnknown70<h4>Background</h4><i>Enterococcus thailandicus</i> has been isolated from animals, fermented foods, wastewater, and humans, but its clinical relevance remains unclear. We investigated the pathogenic potential of <i>E. thailandicus</i> by phenotypic and genomic characterization of two human isolates from acute abdominal infections, complemented by a species-wide comparative genomic analysis of all publicly available <i>E. thailandicus</i> genomes.<h4>Methods</h4>Two <i>E. thailandicus</i> isolates were recovered from patients with acute cholecystitis and small bowel perforation. Species identification was performed by MALDI-TOF MS and ANI analysis, and antimicrobial susceptibility testing was conducted. Whole-genome sequencing (WGS) was performed, followed by <i>in silico</i> analysis of aNew microbes and new infections&lt;i&gt;Enterococcus thailandicus&lt;/i&gt;: Genomic evidence of a potential new player in enterococcal virulence and antimicrobial resistance.PMC12870466IN606B-2022/009PI23/00851IN606A 2023/023CB21/13/00055LA/P/0140/2020UIDB/04378/2020CM23/00104PI21/00704UIDP/04378/2020IN607A 2020/05IN607D 2021/12FI24/00178Velasco DAja-Macaya PPeixe LNovais CFreitas ARFernandez ARodriguez-Pallares SRodriguez-Coello ABeceiro ABou GOuteda-Garcia MAlmeida-Santos ACVazquez-Ucha JCfalse&lt;i&gt;Enterococcus thailandicus&lt;/i&gt;: Genomic evidence of a potential new player in enterococcal virulence and antimicrobial resistance.<h4>Background</h4><i>Enterococcus thailandicus</i> has been isolated from animals, fermented foods, wastewater, and humans, but its clinical relevance remains unclear. We investigated the pathogenic potential of <i>E. thailandicus</i> by phenotypic and genomic characterization of two human isolates from acute abdominal infections, complemented by a species-wide comparative genomic analysis of all publicly available <i>E. thailandicus</i> genomes.<h4>Methods</h4>Two <i>E. thailandicus</i> isolates were recovered from patients with acute cholecystitis and small bowel perforation. Species identification was performed by MALDI-TOF MS and ANI analysis, and antimicrobial susceptibility testing was conducted. Whole-genome sequencing (WGS) was performed, followed by <i>in silico</i> analysis of a2026-01-01T00:00:00Z2026 Apr2026-07-04T03:20:11.915Z2026-07-04T03:13:13.453ZS-EPMC128704664164650810.1016/j.nmni.2026.101711