biostudies-literatureLu SNational Institute of Allergy and Infectious DiseasesIntramural NIH HHSConselho Nacional de Desenvolvimento Científico e TecnológicoCoordination for the improvement of Higher Education PersonnelNational Institute of Allergy and Infectious Diseases Division of Intramural ResearchU.S. Department of Health and Human ServicesFoundation for Research Support of Rio Grande do Sul StateNational Institute of Science and Technology in Molecular EntomologyNational Institutes of Health102600https://www.ebi.ac.uk/biostudies/studies/S-EPMC12872091biostudies-literatureUnknown17(1)The tropical horse tick, Dermacentor nitens, is a one-host hard tick widely distributed across tropical and subtropical regions of the Americas, where it primarily parasitizes horses and occasionally cattle and other livestock. This species is of veterinary importance due to its role as a vector of Babesia caballi, the causative agent of equine piroplasmosis. Despite its significance, genomic resources for D. nitens remain scarce, limiting molecular and functional studies. Here, we present the most comprehensive transcriptome assembly of D. nitens to date, encompassing midgut and ovary transcriptomes during the rapid phase of blood feeding. Using deep sequencing approaches, we identified and annotated a large repertoire of transcripts expressed during blood feeding and reproduction, procesTicks and tick-borne diseasesAn insight into the ovary and midgut transcriptome of Dermacentor nitens tick.PMC1287209121/2551\u20130002221\u20133Z99 AI999999465678/2014\u20139302360/2018\u20132405763/2018\u20132ZIA AI00133788881.153222/2017\u201301Berger Mda Silva Vaz Junior IAndrade-Silva VPerinotto WMSTirloni LLu SRibeiro GBBosio CFMoura IAfalseAn insight into the ovary and midgut transcriptome of Dermacentor nitens tick.The tropical horse tick, Dermacentor nitens, is a one-host hard tick widely distributed across tropical and subtropical regions of the Americas, where it primarily parasitizes horses and occasionally cattle and other livestock. This species is of veterinary importance due to its role as a vector of Babesia caballi, the causative agent of equine piroplasmosis. Despite its significance, genomic resources for D. nitens remain scarce, limiting molecular and functional studies. Here, we present the most comprehensive transcriptome assembly of D. nitens to date, encompassing midgut and ovary transcriptomes during the rapid phase of blood feeding. Using deep sequencing approaches, we identified and annotated a large repertoire of transcripts expressed during blood feeding and reproduction, proces2026-01-01T00:00:00Z2026 Jan2026-07-15T11:58:53.786Z2026-07-04T03:12:28.073ZS-EPMC128720914149624310.1016/j.ttbdis.2025.102600