{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["de Pedro MA"],"pubmed_abstract":["<h4>Background</h4>The effects of menstrual blood-derived mesenchymal stromal cell secretome (S-MenSC) on macrophage polarization remain unclear. This study studied the impact of secretomes from basal MenSCs (S-bMenSCs) and those preconditioned with IFNγ and TNFα (S-pMenSCs) on human monocytes and macrophages <i>in vitro</i>.<h4>Methods</h4>S-MenSCs were used to assess their effects on three stages of monocyte-derived cell maturation: i. monocyte differentiation; ii. polarization of monocyte-derived macrophages (MDMs) toward M1-like or M2-like phenotypes; and iii. reprogramming of pre-polarized M1 or M2 macrophages. Surface markers were analyzed by flow cytometry and cytokine gene expression by RT-qPCR. In addition, a proteomic profiling was performed to identify proteins involved in the o"],"journal":["Frontiers in cell and developmental biology"],"pagination":["1691010"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12872789"],"repository":["biostudies-literature"],"pubmed_title":["Menstrual blood-derived mesenchymal stromal cell secretome modulates macrophage polarization in a preconditioning-dependent manner."],"pmcid":["PMC12872789"],"pubmed_authors":["Gomez-Serrano M","Pulido M","Porschke J","de Pedro MA","Sanchez-Margallo FM","Marchena AM","Graumann J","Pogge von Strandmann E","Reinartz S","Szymanski W","Lopez E","Alvarez V","Gonzalez-Nuno FM"],"additional_accession":[]},"is_claimable":false,"name":"Menstrual blood-derived mesenchymal stromal cell secretome modulates macrophage polarization in a preconditioning-dependent manner.","description":"<h4>Background</h4>The effects of menstrual blood-derived mesenchymal stromal cell secretome (S-MenSC) on macrophage polarization remain unclear. This study studied the impact of secretomes from basal MenSCs (S-bMenSCs) and those preconditioned with IFNγ and TNFα (S-pMenSCs) on human monocytes and macrophages <i>in vitro</i>.<h4>Methods</h4>S-MenSCs were used to assess their effects on three stages of monocyte-derived cell maturation: i. monocyte differentiation; ii. polarization of monocyte-derived macrophages (MDMs) toward M1-like or M2-like phenotypes; and iii. reprogramming of pre-polarized M1 or M2 macrophages. Surface markers were analyzed by flow cytometry and cytokine gene expression by RT-qPCR. In addition, a proteomic profiling was performed to identify proteins involved in the o","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-07-15T06:37:34.39Z","creation":"2026-07-02T03:08:38.322Z"},"accession":"S-EPMC12872789","cross_references":{"pubmed":["41660011"],"doi":["10.3389/fcell.2025.1691010"]}}