biostudies-literatureUnknown14(2)Xiong M<h4>Background</h4>Periodontitis, a chronic inflammatory disease, represents the primary cause of tooth loss in Chinese adults. Wilms tumor 1-associating protein (WTAP) is a key component of the N6-methyladenosine (m6A) methyltransferase complex, and has an unclear role in periodontitis pathogenesis, particularly concerning its regulatory functions in periodontal ligament stem cells (PDLSCs).<h4>Methods</h4>The target gene was identified through the GES260558 dataset and Genecards database. Gene expression was measured using reverse transcription-quantitative PCR (RT-qPCR) and western blot. Periodontitis-derived PDLSCs (P-PDLSCs) were isolated and identified by alkaline phosphatase (ALP) staining, oil red O staining, and flow cytometry. Malondialdehyde (MDA), superoxide dismutase (SOD), anImmunity, inflammation and diseasee70335https://www.ebi.ac.uk/biostudies/studies/S-EPMC12873629biostudies-literatureWTAP Contributes to Periodontitis Pathogenesis by Promoting PDLSC Senescence and Impairing Osteogenic Differentiation via m6A-Dependent Regulation of TP53BP1.PMC12873629Liu YXiong MWang TfalseWTAP Contributes to Periodontitis Pathogenesis by Promoting PDLSC Senescence and Impairing Osteogenic Differentiation via m6A-Dependent Regulation of TP53BP1.<h4>Background</h4>Periodontitis, a chronic inflammatory disease, represents the primary cause of tooth loss in Chinese adults. Wilms tumor 1-associating protein (WTAP) is a key component of the N6-methyladenosine (m6A) methyltransferase complex, and has an unclear role in periodontitis pathogenesis, particularly concerning its regulatory functions in periodontal ligament stem cells (PDLSCs).<h4>Methods</h4>The target gene was identified through the GES260558 dataset and Genecards database. Gene expression was measured using reverse transcription-quantitative PCR (RT-qPCR) and western blot. Periodontitis-derived PDLSCs (P-PDLSCs) were isolated and identified by alkaline phosphatase (ALP) staining, oil red O staining, and flow cytometry. Malondialdehyde (MDA), superoxide dismutase (SOD), an2026-01-01T00:00:00Z2026 Feb2026-07-05T03:08:57.414Z2026-07-05T03:08:02.377ZS-EPMC128736294164151710.1002/iid3.70335