biostudies-literatureUnknown25(2)Jensen PTS-palmitoylation is a poorly understood post-translational modification that is gaining more attention as an essential regulator of cellular processes. The reversible nature of S-palmitoylation may allow for fine-tuned control of cellular events and adaptation to stimuli. The detection of S-palmitoylated proteins and peptides includes the Acyl-Biotin Exchange (ABE) method, Acyl resin-assisted Capture (Acyl-RAC), metabolic labelling, and derivatives thereof. We present a novel method of enrichment of S-palmitoylated peptides termed SDC Acid Precipitation Enrichment (SDC-ACE). Here, S-palmitoylated peptides are enriched by taking advantage of their co-precipitation with Sodium deoxycholate (SDC) under acidic conditions, allowing easy and fast separation of lipidated peptides from the sample Molecular & cellular proteomics : MCP101218https://www.ebi.ac.uk/biostudies/studies/S-EPMC12874130biostudies-literatureEnrichment of Cysteine S-palmitoylated Peptides Using Sodium Deoxycholate Acid Precipitation.PMC12874130Jensen PTRhodes CJPalmisano GLarsen MRfalseEnrichment of Cysteine S-palmitoylated Peptides Using Sodium Deoxycholate Acid Precipitation.S-palmitoylation is a poorly understood post-translational modification that is gaining more attention as an essential regulator of cellular processes. The reversible nature of S-palmitoylation may allow for fine-tuned control of cellular events and adaptation to stimuli. The detection of S-palmitoylated proteins and peptides includes the Acyl-Biotin Exchange (ABE) method, Acyl resin-assisted Capture (Acyl-RAC), metabolic labelling, and derivatives thereof. We present a novel method of enrichment of S-palmitoylated peptides termed SDC Acid Precipitation Enrichment (SDC-ACE). Here, S-palmitoylated peptides are enriched by taking advantage of their co-precipitation with Sodium deoxycholate (SDC) under acidic conditions, allowing easy and fast separation of lipidated peptides from the sample 2025-01-01T00:00:00Z2025 Oct2026-07-15T06:19:01.773Z2026-06-30T03:23:05.881ZS-EPMC128741304110948010.1016/j.mcpro.2025.101218