{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tan J"],"funding":["Shanghai Basic Research Pioneer Project","Chinese Academy of Sciences","Shanghai Key Laboratory of Aging Studies","National Natural Science Foundation of China","Shanghai Municipal Youth Science and Technology Star Project","Youth Innovation Promotion Association of the Chinese Academy of Sciences"],"pagination":["45774-45784"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12874534"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["147(49)"],"pubmed_abstract":["Radical reactions offer transformative potential in biological contexts but remain constrained by poor selectivity and off-target reactivity. We address these limitations through visible-light photocatalytic generation of diaryl ketyl radicals from benzophenones. This strategy circumvents traditional UV excitation pathways by suppressing triplet diradical formation─which drives nonspecific [2 + 2] cycloadditions and H atom abstraction─in favor of bioorthogonal radical-radical coupling. Our platform enables precise live-cell protein labeling with minimal cytotoxicity, including in sensitive primary neuronal cultures, and achieves site-specific modification via genetically incorporated benzophenone-based unnatural amino acids Bpa. The spatial selectivity of this approach exceeds conventional"],"journal":["Journal of the American Chemical Society"],"pubmed_title":["Bioorthogonal Photocatalytic Protein Labeling and Cross-Linking Enabled by Stabilized Ketyl Radicals."],"pmcid":["PMC12874534"],"funding_grant_id":["XDB1060000","22277133","2023266","19DZ2260400","22337005"],"pubmed_authors":["Che Q","Qi L","Li Y","Hao K","Wang R","Tan J","Zhang Y","Chen Y","Xie S","Yuan Y"],"additional_accession":[]},"is_claimable":false,"name":"Bioorthogonal Photocatalytic Protein Labeling and Cross-Linking Enabled by Stabilized Ketyl Radicals.","description":"Radical reactions offer transformative potential in biological contexts but remain constrained by poor selectivity and off-target reactivity. We address these limitations through visible-light photocatalytic generation of diaryl ketyl radicals from benzophenones. This strategy circumvents traditional UV excitation pathways by suppressing triplet diradical formation─which drives nonspecific [2 + 2] cycloadditions and H atom abstraction─in favor of bioorthogonal radical-radical coupling. Our platform enables precise live-cell protein labeling with minimal cytotoxicity, including in sensitive primary neuronal cultures, and achieves site-specific modification via genetically incorporated benzophenone-based unnatural amino acids Bpa. The spatial selectivity of this approach exceeds conventional","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-07-15T09:12:24.135Z","creation":"2026-07-02T03:08:52.284Z"},"accession":"S-EPMC12874534","cross_references":{"pubmed":["41315065"],"doi":["10.1021/jacs.5c18652"]}}