{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16"],"submitter":["Wang J"],"pubmed_abstract":["<h4>Introduction</h4>Osteoarthritis (OA) is a degenerative joint disease marked by chronic inflammation, extracellular matrix degradation, and dysregulated cell death. The roles of apoptosis-, autophagy-, and ferroptosis-related genes in OA pathogenesis remain unclear.<h4>Methods</h4>Integrated bioinformatics analyses were conducted on public GEO datasets to identify apoptosis-autophagy-ferroptosis-related genes (AAFRGs). TLR3 and GLUL were identified using LASSO, random forest, and SVM-RFE algorithms. Immune infiltration analysis, immunohistochemistry, and functional assays in human chondrocytes were performed, and ACLT-induced rat OA models were used for <i>in vivo</i> validation.<h4>Results</h4>TLR3 was upregulated and associated with pro-inflammatory immune cells, while GLUL was downre"],"journal":["Frontiers in immunology"],"pagination":["1650375"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12875911"],"repository":["biostudies-literature"],"pubmed_title":["TLR3 and GLUL orchestrate inflammatory and homeostatic imbalance in osteoarthritis."],"pmcid":["PMC12875911"],"pubmed_authors":["Peng P","Xu S","Qin Y","Wang J","Chen B","Wang K"],"additional_accession":[]},"is_claimable":false,"name":"TLR3 and GLUL orchestrate inflammatory and homeostatic imbalance in osteoarthritis.","description":"<h4>Introduction</h4>Osteoarthritis (OA) is a degenerative joint disease marked by chronic inflammation, extracellular matrix degradation, and dysregulated cell death. The roles of apoptosis-, autophagy-, and ferroptosis-related genes in OA pathogenesis remain unclear.<h4>Methods</h4>Integrated bioinformatics analyses were conducted on public GEO datasets to identify apoptosis-autophagy-ferroptosis-related genes (AAFRGs). TLR3 and GLUL were identified using LASSO, random forest, and SVM-RFE algorithms. Immune infiltration analysis, immunohistochemistry, and functional assays in human chondrocytes were performed, and ACLT-induced rat OA models were used for <i>in vivo</i> validation.<h4>Results</h4>TLR3 was upregulated and associated with pro-inflammatory immune cells, while GLUL was downre","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-07-05T03:14:55.4Z","creation":"2026-07-05T03:08:42.03Z"},"accession":"S-EPMC12875911","cross_references":{"pubmed":["41660607"],"doi":["10.3389/fimmu.2025.1650375"]}}